Transcriptomics,Multiomics

Dataset Information

6

Effect of cajanin stilbene acid and its synthetic derivatives on MCF-7 breast cancer cells


ABSTRACT: Cajanin stilbene acid (CSA) was previously shown to induce cytotoxicity against cancer cells. We have employed microarray gene expression profiling to identify deregulated genes in MCF-7 breast cancer cells upon treatment with CSA and its synthetic derivatives (CSA6, CSA9, CSA19). TGM2 gene encoding transglutaminase 2 was commonly deregulated after treatment with all four CSA compounds. All compounds revealed strong effects on cell cycle progression and DNA damage response. Promoter motif analysis of the deregulated genes further supported the microarray results emphasizing the relevance of transcription factors regulating cell cycle and proliferation, MYC being among the most pronounced ones. Interestingly, cellular movement was among the top five affected cellular functions after treatment with the four derivatives. Gene expression profiling study with Whole Human Genome RNA chips (8×60K Agilent) was performed in MCF-7 breast cancer cells upon treatment with CSA and its synthetic derivatives (CSA6, CSA9, CSA19). Twelve chips were used; control (x2), CSA (x2), control (x2), CSA6 (x2), CSA9 (x2), CSA19 (x2). MCF-7 cells were treated with the compounds (IC50 concentrations) for 72 hours or left untreated (DMSO control). Two independent experiments were performed for each treatment.

OTHER RELATED OMICS DATASETS IN: PRJNA271005

ORGANISM(S): Homo sapiens  

SUBMITTER: Benjamin Wiench   Thomas Efferth  Yuangang Zu  Yujie Fu  Onat Kadioglu 

PROVIDER: E-GEOD-64430 | ArrayExpress | 2015-02-27

SECONDARY ACCESSION(S): GSE64430PRJNA271005

REPOSITORIES: GEO, ArrayExpress

Dataset's files

Source:
Action DRS
E-GEOD-64430.additional.1.zip Other
E-GEOD-64430.idf.txt Idf
E-GEOD-64430.processed.1.zip Processed
E-GEOD-64430.raw.1.zip Raw
E-GEOD-64430.sdrf.txt Txt
Items per page:
1 - 5 of 5
altmetric image

Publications

Synthetic cajanin stilbene acid derivatives inhibit c-MYC in breast cancer cells.

Kadioglu Onat O   Fu Yujie Y   Wiench Benjamin B   Zu Yuangang Y   Efferth Thomas T  

Archives of toxicology 20150226 3


In the present study, we investigated the activity and modes of action of cajanin stilbene acid (CSA) and its derivatives in terms of cytotoxicity, gene expression profile, and transcription factor activity. XTT assays on MCF7 cells were performed upon treatment with CSA or derivatives. After the determination of IC50 values, gene expression profiling was performed with Agilent microarray experiments. Deregulated genes were determined with Chipster software, pathway and functional analyses were  ...[more]

Similar Datasets

| GSE64430 | GEO
2020-01-01 | S-EPMC7594071 | BioStudies
2017-01-01 | S-EPMC5487404 | BioStudies
2015-01-01 | S-EPMC4791264 | BioStudies
1000-01-01 | S-EPMC5630666 | BioStudies
2018-01-01 | S-EPMC5772076 | BioStudies
2010-05-15 | E-GEOD-16737 | ArrayExpress
1000-01-01 | S-EPMC6278399 | BioStudies
2020-01-01 | S-EPMC7321239 | BioStudies
2015-01-07 | E-GEOD-58574 | ArrayExpress