Dataset Information


Sensing the end: how TUT7 controls the fate of precursor microRNAs by uridylation

ABSTRACT: Terminal uridylyl transferases (TUTs) function as integral regulators of microRNA (miRNA) biogenesis by modifying the end structure of precursor miRNA (pre-miRNA). Using biochemistry and deep sequencing techniques, we here investigate the mechanism how human TUT7 recognizes and uridylates pre-miRNAs. We show that the overhang of a pre-miRNA is the key structural element that TUT7 and its paralogues, TUT4 and TUT2, recognize. For group II pre-miRNAs which have a 1 nt 3’ overhang, TUT7 restores the canonical end structure (2 nt 3’ overhang) by mono-uridylation, and thereby promotes miRNA biogenesis. Interestingly, once the 3’ end is receded into the stem (3’ trimmed pre-miRNAs such as Ago-cleaved-pre-miRNA), TUT7 effectively generates an oligo-U tail that consequently leads to degradation. Our single-molecule study further suggests that a distributive mode is employed for both pathways, but the overhang length determines the frequency of TUT7-RNA interaction. Our results explain how TUT7 and TUT4 differentiate pre-miRNA species and reveal a role for TUT7 and TUT4 in the oligo-uridylation and removal of defective pre-miRNAs. HeLa cells were knocked down of control or TUT2/4/7, then total RNAs were prepared for RNA-seq

ORGANISM(S): Homo sapiens  

SUBMITTER: Boseon Kim   Hyeshik Chang  V N Kim 

PROVIDER: E-GEOD-64482 | ArrayExpress | 2015-04-30



Similar Datasets

| GSE64482 | GEO
2014-12-04 | E-GEOD-59626 | ArrayExpress
2014-12-04 | E-GEOD-59627 | ArrayExpress
2012-10-16 | E-GEOD-35835 | ArrayExpress
2012-10-16 | E-GEOD-40228 | ArrayExpress
| GSE59627 | GEO
2013-12-01 | E-GEOD-52575 | ArrayExpress
2019-01-14 | E-MTAB-7065 | ArrayExpress
| GSE111152 | GEO
| PRJNA255774 | ENA