Transcriptomics

Dataset Information

298

Conversion of MyoD to a neurogenic factor: binding site specificity determines lineage [RNA-seq]


ABSTRACT: MyoD and NeuroD2 are master regulators of myogenesis and neurogenesis and bind to a "shared" E-box sequence (CAGCTG) and a "private" sequence (CAGGTG or CAGATG, respectively). To determine whether private-site recognition is sufficient to confer lineage-specification, we generated a MyoD-mutant with the DNA binding specificity of NeuroD2. Our results demonstrate that redirecting MyoD binding from MyoD-private sites to NeuroD2-private sites, despite preserved binding to the MyoD/NeuroD2-shared sites, is sufficient to change MyoD from a master regulator of myogenesis to a master regulator of neurogenesis. RNA-seq profiling of mouse P19 cells transfected with MyoD, NeuroD2 and chimera mutants. The chimeric mutants are MyoD with the bHLH domain replaced with the NeuroD2 bHLH domain.

ORGANISM(S): Mus musculus  

SUBMITTER: Stephen Tapscott   Abraham Fong  Jun W Zhong  Zizhen Yao  Zizhen yao 

PROVIDER: E-GEOD-64622 | ArrayExpress | 2015-07-31

SECONDARY ACCESSION(S): SRP051986GSE64622PRJNA272314

REPOSITORIES: GEO, ArrayExpress, ENA

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