Transcriptomics,Multiomics

Dataset Information

11

MicroRNA profiling in the zoonotic parasite Echinococcus canadensis using a high-throughput approach


ABSTRACT: microRNAs (miRNAs), a class of small non-coding RNAs, are key regulators of gene expression at post-transcriptional level and play essential roles in fundamental biological processes such as development and metabolism. Here, we perform a comprehensive analysis of miRNAs in the zoonotic parasite E. canadensis G7, one of the causative agents of the neglected disease cystic echinococcosis. Small RNA libraries from protoscoleces and cyst walls of E. canadensis G7 and protoscoleces of E. granulosus sensu stricto G1 were sequenced using Illumina technology. As a result, we found transcriptional evidence of 37 miRNAs thus expanding the miRNA repertoire of E. canadensis G7. Differential expression analysis showed significant regulated miRNAs between life cycle stages of E. canadensis G7. We confirmed the remarkable loss of conserved miRNA families in E. canadensis, reflecting their low morphological complexity and high adaptation to parasitism. This study will provide valuable information for better understanding the complex biology of this parasite and could help to find new potential targets for therapy and/or diagnosis. Small RNA libraries from protoscoleces and cyst walls of E. canadensis G7 and protoscoleces of E. granulosus sensu stricto G1 were sequenced using Illumina technology. For each sample type, two libraries were constructed from two independent samples in order to have biological replicates.

OTHER RELATED OMICS DATASETS IN: PRJNA271689

ORGANISM(S): Echinococcus Canadensis Echinococcus Granulosus

SUBMITTER: Natalia Macchiaroli   Marcela Cucher  Laura Kamenetzky  Magdalena Zarowiecki  Mara C Rosenzvit  Lucas Maldonado 

PROVIDER: E-GEOD-64705 | ArrayExpress | 2015-02-09

SECONDARY ACCESSION(S): SRP051731GSE64705PRJNA271689

REPOSITORIES: GEO, ArrayExpress, ENA

Dataset's files

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E-GEOD-64705.additional.1.zip Other
E-GEOD-64705.idf.txt Idf
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Publications

microRNA profiling in the zoonotic parasite Echinococcus canadensis using a high-throughput approach.

Macchiaroli Natalia N   Cucher Marcela M   Zarowiecki Magdalena M   Maldonado Lucas L   Kamenetzky Laura L   Rosenzvit Mara Cecilia MC  

Parasites & vectors 20150206


<h4>Background</h4>microRNAs (miRNAs), a class of small non-coding RNAs, are key regulators of gene expression at post-transcriptional level and play essential roles in fundamental biological processes such as development and metabolism. The particular developmental and metabolic characteristics of cestode parasites highlight the importance of studying miRNA gene regulation in these organisms. Here, we perform a comprehensive analysis of miRNAs in the parasitic cestode Echinococcus canadensis G7  ...[more]

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