Transcriptomics

Dataset Information

32

Expression data from RhoA-null and wildtype keratinocytes


ABSTRACT: Using mice with a keratinocyte-restricted deletion of the RhoA gene, we explored its role in the regulation of gene expression in vivo and in vitro RNA was isolated from RhoA-null or wildtype keratinocytes, in two different conditions, freshly isolated (in vivo) or cultured (in vitro), using the RNeasy kit (Qiagen, Germany). Hybridization to GeneChip Mouse Genome 430 2.0 Array (Affymetrix, Santa Clara, CA) was carried out at the Copenhagen University Hospital Microarray Center using standard protocols. 2 biological replicates were analyzed for each in vivo genotype (2 samples from RhoA-null, 2 from wildtype), while 3 replicates for each in vitro genotype.

ORGANISM(S): Mus musculus  

SUBMITTER: Alberto Garcia Mariscal  Alberto G Mariscal   Karine Peyrollier    

PROVIDER: E-GEOD-64714 | ArrayExpress | 2015-01-10

SECONDARY ACCESSION(S): GSE64714PRJNA271709

REPOSITORIES: GEO, ArrayExpress

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