Transcriptomics

Dataset Information

6

Specific gene expression related to epiblast development


ABSTRACT: To explore genes involved in epiblast development, we have employed whole genome microarray expression profiling as a discovery platform to identify genes. Embryoid body (EB) formation induces embryonic stem (ES) cells to differentiate into germ layers via epiblasts and extra-embryonic tissues, whereas ES cells give rise only to extra-embryonic tissues in monolayer culture. We compared gene expression profiles of ES cells in each differentiation culture methods at day 1-3 or 4 of differentiation. The expression of organizer related genes (Lhx1, Cer1 and Foxa2) was specifically induced at day 3 of EBs, but not in monolayer culture. In epiblast model cells, the overexpression of Lhx1 induced gene expression of organizer and meso-endodermal markers, suggested that Lhx1 was related to epiblast differentiation. Gene expression related epiblast development was measured at undifferentiated state and day 1-3 or 4 of differentiation. Two independent experiments were performed at each time using different people for each experiment.

ORGANISM(S): Mus musculus  

SUBMITTER: Ichiro Hisatome   Yasuaki Shirayoshi  Akira Hasegawa  Nobuhito Ikeda 

PROVIDER: E-GEOD-64715 | ArrayExpress | 2015-04-01

SECONDARY ACCESSION(S): GSE64715PRJNA271729

REPOSITORIES: GEO, ArrayExpress

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