Transcriptomics

Dataset Information

0

MicroRNA expression profiling of different T cell subset in human tonsils


ABSTRACT: Tight control of follicular helper T(Tfh) cells is required for optimal maturation of the germinal center (GC) response. The molecular mechansims controlling Tfh cell differentiation remain incompletely understood. Here we sought to to identfiy miRNAs that might regulate Tfh cell development and/or function. To identfiy miRNAs that are differentially expressed in Tfh cells, we performed Agilent microRNA microarray analysis comparing Tfh cells with the other main T cell subsets (naive T cells, non-Tfh effector or non-Tfh memory T cells, CD57+ Tfh cells and CD57- Tfh cells). RNA was extracted from 4 separate human tonsils (biological replicates) using mirVANA RNA isolation kit (Ambion) according to the manufacturer’s protocol. Each biological sample was analysed individually. Live lymphocytes were sorted based on negative 7AAD staining, and thefollowing four subsets were purified: Naive T cells (CD4+ CD45RO-); CD57+ Tfh cells (CD4+ CD45RO+ PD-1_high CXCR5_high CD57+); CD57- Tfh cells (CD4+ CD45RO+ PD-1_high CXCR5_high CD57-) and non-Tfh effector or memory T cells (CD4+ CD45RO+ PD-1_neg CXCR5_neg). RNA quantity and quality was determined using a Nanodrop 1000 and an Agilent 2100 Bioanalyzer respectively. Hybridization on Agilent Human miRNA Microarray (V1) Kit, 8x15K and was performed at The Ramaciotti Center for Genomics (University of New South Wales, Australia). The mean of 3-4 biological replicates from each population of each population were calculated and values were plotted using Prism.

ORGANISM(S): Homo sapiens  

SUBMITTER: Carola G Vinuesa  Alvin Pratama   Monika Srivastava   Robert Rigby    

PROVIDER: E-GEOD-64833 | ArrayExpress | 2015-01-10

SECONDARY ACCESSION(S): GSE64833PRJNA272140

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

Sorry, this publication's infomation has not been loaded in the Indexer, please go directly to PUBMED or Altmetric.

Similar Datasets

2016-04-01 | E-GEOD-73927 | ArrayExpress
2013-11-12 | E-GEOD-50391 | ArrayExpress
| GSE62961 | GEO
| GSE98347 | GEO
| GSE106455 | GEO
2011-06-02 | E-GEOD-24574 | ArrayExpress
2012-02-10 | E-MEXP-2578 | ArrayExpress
2014-01-22 | E-GEOD-51531 | ArrayExpress
2011-06-02 | GSE24574 | GEO
2015-05-25 | BIOMD0000000625 | BioModels Database