Transcriptomics

Dataset Information

32

Temporal Changes in Rat Liver Gene Expression after Acute Cadmium and Chromium Exposure


ABSTRACT: U.S. Service Members and civilians are at risk of exposure to a variety of environmental health hazards throughout their normal duty activities and in industrial occupations. Metals are widely used in large quantities in a number of industrial processes and are a common environmental toxicant, which increases the possibility of being exposed at toxic levels. While metal toxicity has been widely studied, the exact mechanisms of toxicity remain unclear. In order to further elucidate these mechanisms and identify candidate biomarkers, rats were exposed via a single intraperitoneal injection to three concentrations of CdCl2 and Na2Cr2O7, with livers harvested at 1, 3, or 7 days after exposure. Cd and Cr accumulated in the liver at 1 day post exposure. Cd levels remained elevated over the length of the experiment, while Cr levels declined. Metal exposures induced ROS, including hydroxyl radical (•OH), resulting in DNA strand breaks and lipid peroxidation. Interestingly, ROS and cellular damage appeared to increase with time post-exposure in both metals, despite declines in Cr levels. Differentially expressed genes were identified via microarray analysis. Both metals perturbed gene expression in pathways related to oxidative stress, metabolism, DNA damage, cell cycle, and inflammatory response. This work provides insight into the temporal effects and mechanistic pathways involved in acute metal intoxication, leading to the identification of candidate biomarkers. Rats (5 per group) were exposed via a single intraperitoneal injection to three concentrations of CdCl2, Na2Cr2O7, or vehicle control,with livers harvested at 1, 3, or 7 days after exposure. Only the Cd mid dose and Cr high dose were selected for microarray analysis.

ORGANISM(S): Rattus norvegicus  

SUBMITTER: Christine E Baer  John A Lewis   Jonathan D Stallings   Stephen S Leonard   Valerie C Minarchick   William E Dennis   Michael S Madejczyk   Michael Scott Madejczyk   David A Jackson    

PROVIDER: E-GEOD-65198 | ArrayExpress| 2015-05-23

SECONDARY ACCESSION(S): GSE65198PRJNA273384

REPOSITORIES: GEO, ArrayExpress

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Publications

Temporal changes in rat liver gene expression after acute cadmium and chromium exposure.

Madejczyk Michael S MS   Baer Christine E CE   Dennis William E WE   Minarchick Valerie C VC   Leonard Stephen S SS   Jackson David A DA   Stallings Jonathan D JD   Lewis John A JA  

PloS one 20150519 5


U.S. Service Members and civilians are at risk of exposure to a variety of environmental health hazards throughout their normal duty activities and in industrial occupations. Metals are widely used in large quantities in a number of industrial processes and are a common environmental toxicant, which increases the possibility of being exposed at toxic levels. While metal toxicity has been widely studied, the exact mechanisms of toxicity remain unclear. In order to further elucidate these mechanis  ...[more]

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