Transcriptomics

Dataset Information

5

Transcription profiling by array of Arabidopsis heterozygous mutant for gh3.5 after inoculation with Pseudomonas syringae pv tomato DC3000(avrRpt2) against wild type counterparts and uninfected controls


ABSTRACT: To reveal the underlying molecular mechanism of GH3.5 action in modulating the SA and auxin pathways, we performed transcriptional profiling of gh3.5-1D plants after infection with or without Pst DC3000(avrRpt2) on a global scale using the Affymetrix Arabidopsis ATH1 GeneChip Experiment Overall Design: Each 3 leaves of plants of 5-week-old wild type Columbia-0 and mutant gh3.5-1D (heterozygous) were inoculated with Pst DC3000(avrRpt2)at 105 cfu mL-1. Leaves were harvested at 0 h (uninoculated) and 48 hours after inoculation. Three biological repeats were performed on Columbia-0 (wild-type, Col-0) and gh3.5-1D (mutant) after infection with or without Pst DC3000(avrRpt2), respectively.

REANALYSED by: E-GEOD-6556

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Li Qun  

PROVIDER: E-GEOD-6556 | ArrayExpress | 2008-06-14

SECONDARY ACCESSION(S): GSE6556PRJNA98769

REPOSITORIES: GEO, ArrayExpress

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Publications

Dual regulation role of GH3.5 in salicylic acid and auxin signaling during Arabidopsis-Pseudomonas syringae interaction.

Zhang Zhongqin Z   Li Qun Q   Li Zhimiao Z   Staswick Paul E PE   Wang Muyang M   Zhu Ying Y   He Zuhua Z  

Plant physiology 20070817 2


Salicylic acid (SA) plays a central role in plant disease resistance, and emerging evidence indicates that auxin, an essential plant hormone in regulating plant growth and development, is involved in plant disease susceptibility. GH3.5, a member of the GH3 family of early auxin-responsive genes in Arabidopsis (Arabidopsis thaliana), encodes a protein possessing in vitro adenylation activity on both indole-3-acetic acid (IAA) and SA. Here, we show that GH3.5 acts as a bifunctional modulator in bo  ...[more]

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