Transcriptomics

Dataset Information

5

Transcriptional correlates of FOXP3 expression in suppressive and non-suppressive primary human Treg cell clones.


ABSTRACT: Analysis of the transcriptional correlates of FOXP3 expression in suppressive and non-suppressive primary human Treg cell clones. Individual CD4+CD25High or Cd4+CD25- T cells were isolated from human PBMCs and expanded in vitro. After 3 weeks of expansion, individual clones were analysed for FOXP3 expression and in vitro suppressive activity against freshly sorted allogeneic effector T cells. This study analyses the total RNA isolated from FOXP3+ clones with suppressive potency to their non-suppressive counterparts. The resutls of this study should provide insights into the molecular pathways linking FOXP3 expression to distinct aspects of Treg phenotype and function. Total RNA obtained from individual clones of primary human regulatory and effector CD4+T cells.

ORGANISM(S): Homo sapiens  

SUBMITTER: Eva d'Hennezel   Khalid Bin Dhuban  Ciriaco Piccirillo  Ola Larsson 

PROVIDER: E-GEOD-65650 | ArrayExpress | 2015-05-06

SECONDARY ACCESSION(S): GSE65650PRJNA274579

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

Coexpression of TIGIT and FCRL3 identifies Helios+ human memory regulatory T cells.

Bin Dhuban Khalid K   d'Hennezel Eva E   Nashi Emil E   Bar-Or Amit A   Rieder Sadiye S   Shevach Ethan M EM   Nagata Satoshi S   Piccirillo Ciriaco A CA  

Journal of immunology (Baltimore, Md. : 1950) 20150311 8


Two distinct subsets of CD4(+)Foxp3(+) regulatory T (Treg) cells have been described based on the differential expression of Helios, a transcription factor of the Ikaros family. Efforts to understand the origin and biological roles of these Treg populations in regulating immune responses have, however, been hindered by the lack of reliable surface markers to distinguish and isolate them for subsequent functional studies. Using a single-cell cloning strategy coupled with microarray analysis of di  ...[more]

Similar Datasets

2018-05-18 | PXD005477 | Pride
2018-05-18 | PXD007744 | Pride
2013-03-08 | E-GEOD-41087 | ArrayExpress
2010-04-12 | E-GEOD-17580 | ArrayExpress
2012-10-03 | E-GEOD-39529 | ArrayExpress
2015-11-01 | E-GEOD-63455 | ArrayExpress
| GSE84919 | GEO
2018-05-18 | PXD007745 | Pride
2015-12-10 | E-GEOD-68621 | ArrayExpress
2017-02-15 | PXD001906 | Pride