Transcriptomics,Multiomics

Dataset Information

5

Complete Haplotype Phasing of the MHC and KIR Loci with Targeted HaploSeq

ABSTRACT: Rapid advances in biochemical technologies have enabled several strategies for typing candidate HLA alleles, but linking them into a single MHC haplotype structure remains challenging. Here we have developed a multi-loci haplotype phasing technique and demonstrate its utility towards phasing of MHC and KIR loci in human samples. We accurately (~99%) reconstruct the complete haplotypes for over 90% of sequence variants spanning the 4-megabase region of these two loci. By haplotyping a majority of coding and non-coding alleles at the MHC and KIR loci in a single assay, this method has the potential to assist transplantation matching and facilitate investigation of the genetic basis of human immunity and disease. Complete haplotype phasing of 2 loci (MHC and KIR) in 1 human cell line.

OTHER RELATED OMICS DATASETS IN: PRJNA274802

ORGANISM(S): Homo Sapiens

SUBMITTER: Anthony D Schmitt   Bing Ren  Anthony Schmitt  Siddarth Selvaraj 

PROVIDER: E-GEOD-65726 | ArrayExpress | 2016-02-01

REPOSITORIES: ArrayExpress

Json Xml

Dataset's files

Source:
Action DRS
E-GEOD-65726.additional.1.zip Other
E-GEOD-65726.idf.txt Idf
E-GEOD-65726.sdrf.txt Txt
Items per page:
1 - 3 of 3
altmetric image

Publications

Complete haplotype phasing of the MHC and KIR loci with targeted HaploSeq.

Selvaraj Siddarth S   Schmitt Anthony D AD   Dixon Jesse R JR   Ren Bing B  

BMC genomics 20151105

<h4>Background</h4>The MHC and KIR loci are clinically relevant regions of the genome. Typing the sequence of these loci has a wide range of applications including organ transplantation, drug discovery, pharmacogenomics and furthering fundamental research in immune genetics. Rapid advances in biochemical and next-generation sequencing (NGS) technologies have enabled several strategies for precise genotyping and phasing of candidate HLA alleles. Nonetheless, as typing of candidate HLA alleles alo  ...[more]

Similar Datasets

Unknown Complete Haplotype Phasing of the MHC and KIR Loci with Targeted HaploSeq
| E-GEOD-65726 | BioStudies
Unknown Complete Haplotype Phasing of the MHC and KIR Loci with Targeted HaploSeq
2016-02-01 | GSE65726 | GEO
Unknown Complete haplotype phasing of the MHC and KIR loci with targeted HaploSeq.
| S-EPMC4636068 | BioStudies
Unknown,Multiomics Homo sapiens
| PRJNA274802 | ENA
Unknown The LRC haplotype project: a resource for killer immunoglobulin-like receptor-linked association studies.
| S-EPMC2734079 | BioStudies
Unknown Plasticity in the organization and sequences of human KIR/ILT gene families.
| S-EPMC18309 | BioStudies
Unknown Estimation of German KIR Allele Group Haplotype Frequencies.
| S-EPMC7080815 | BioStudies
Unknown A Detailed View of KIR Haplotype Structures and Gene Families as Provided by a New Motif-Based Multiple Sequence Alignment.
| S-EPMC7708349 | BioStudies
Unknown A small, variable, and irregular killer cell Ig-like receptor locus accompanies the absence of MHC-C and MHC-G in gibbons.
| S-EPMC3017126 | BioStudies
Unknown Meiotic recombination generates rich diversity in NK cell receptor genes, alleles, and haplotypes.
| S-EPMC2675964 | BioStudies