Dataset Information


Complete Haplotype Phasing of the MHC and KIR Loci with Targeted HaploSeq

ABSTRACT: Rapid advances in biochemical technologies have enabled several strategies for typing candidate HLA alleles, but linking them into a single MHC haplotype structure remains challenging. Here we have developed a multi-loci haplotype phasing technique and demonstrate its utility towards phasing of MHC and KIR loci in human samples. We accurately (~99%) reconstruct the complete haplotypes for over 90% of sequence variants spanning the 4-megabase region of these two loci. By haplotyping a majority of coding and non-coding alleles at the MHC and KIR loci in a single assay, this method has the potential to assist transplantation matching and facilitate investigation of the genetic basis of human immunity and disease. Complete haplotype phasing of 2 loci (MHC and KIR) in 1 human cell line.


ORGANISM(S): Homo Sapiens

SUBMITTER: Anthony D Schmitt   Bing Ren  Anthony Schmitt  Siddarth Selvaraj 

PROVIDER: E-GEOD-65726 | ArrayExpress | 2016-02-01


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Complete haplotype phasing of the MHC and KIR loci with targeted HaploSeq.

Selvaraj Siddarth S   Schmitt Anthony D AD   Dixon Jesse R JR   Ren Bing B  

BMC genomics 20151105

<h4>Background</h4>The MHC and KIR loci are clinically relevant regions of the genome. Typing the sequence of these loci has a wide range of applications including organ transplantation, drug discovery, pharmacogenomics and furthering fundamental research in immune genetics. Rapid advances in biochemical and next-generation sequencing (NGS) technologies have enabled several strategies for precise genotyping and phasing of candidate HLA alleles. Nonetheless, as typing of candidate HLA alleles alo  ...[more]

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