Transcriptomics

Dataset Information

0

Integrative analysis of RNA, translation and protein levels reveals distinct regulatory variation across humans


ABSTRACT: Elucidating the extent and consequences of genetic differences between humans is essential for understanding phenotypic diversity and personalized medicine. Although variation in RNA levels, transcription factor binding and chromatin have been explored, little is known about global variation in translation and its genetic determinants among humans. We used ribosome profiling, RNA sequencing, and mass spectrometry to perform an integrated analysis in lymphoblastoid cell lines from a diverse group of individuals. We find significant differences in RNA levels, translation, and protein abundance suggesting diverse mechanisms of personalized gene expression control. Combined analysis of RNA expression and ribosome occupancy improves the identification of individual protein level differences. Finally, we identify genetic differences that specifically modulate ribosome occupancy - many of these differences lie close to start codons and upstream ORFs. Our results reveal a new level of gene expression variation among humans and indicate that genetic variants can cause changes in protein levels through effects on translation. Ribosome profiling and RNA sequencing experiments from human lymphoblastoid cells

ORGANISM(S): Homo sapiens  

SUBMITTER: Carlos L Araya  Fabian Grubert   Michael P Snyder   Bilal Alsallakh   Hua Tang   Hagen Tilgner   Emiliano Ricci   Elif Sarinay Cenik   Sophie I Candille   Damek Spacek   Gun W Byeon   Can Cenik   Jason A Reuter    

PROVIDER: E-GEOD-65912 | ArrayExpress | 2015-02-14

SECONDARY ACCESSION(S): SRP055009GSE65912PRJNA275386

REPOSITORIES: GEO, ArrayExpress, ENA

altmetric image

Publications

Sorry, this publication's infomation has not been loaded in the Indexer, please go directly to PUBMED or Altmetric.

Similar Datasets

2013-12-19 | E-GEOD-53313 | ArrayExpress
2013-09-22 | E-GEOD-47558 | ArrayExpress
2016-07-03 | E-GEOD-68265 | ArrayExpress
2012-07-26 | E-GEOD-37744 | ArrayExpress
2013-09-26 | E-GEOD-51164 | ArrayExpress
2015-04-10 | E-GEOD-60752 | ArrayExpress
2016-08-08 | E-GEOD-83616 | ArrayExpress
2016-01-13 | E-GEOD-72899 | ArrayExpress
2015-01-27 | E-GEOD-55195 | ArrayExpress
2016-07-01 | E-GEOD-75947 | ArrayExpress