Dataset Information


ChIPseq analysis of MYST5 (cg1894) in Drosophila S2 cells

ABSTRACT: We report here the genome-wide localization of the histone acetyltransferase MYST5 (cg1984) in Drosophila. ChIP-seq analysis was performed with two anti-MYST5 antibodies in S2 cells. We found MYST5 to bind to the promoters of actively transcribed genes. MYST5 furthermore showed extensive colocalization with boundary/ insulator factors, including Chriz/ Chromator, CP190, dCTCF and BEAF-32, which mediate the organization of the genome into functionally distinct topological domains. Altogether, our data suggest a broad role for MYST5 both in gene-specific transcriptional regulation and in the organization of the genome into chromatin domains. Examination of genome-wide MYST5 localization in S2 cells

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: Ann E. Ehrenhofer-Murray   Ann Ehrenhofer-Murray 

PROVIDER: E-GEOD-66212 | ArrayExpress | 2016-02-23



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Widespread colocalization of the Drosophila histone acetyltransferase homolog MYST5 with DREF and insulator proteins at active genes.

Heseding Christiane C   Saumweber Harald H   Rathke Christina C   Ehrenhofer-Murray Ann E AE  

Chromosoma 20160219 1

MYST family histone acetyltransferases play important roles in gene regulation. Here, we have characterized the Drosophila MYST histone acetyltransferase (HAT) encoded by cg1894, whose closest homolog is Drosophila MOF, and which we have termed MYST5. We found it localized to a large number of interbands as well as to the telomeres of polytene chromosomes, and it showed strong colocalization with the interband protein Z4/Putzig and RNA polymerase II. Accordingly, genome-wide location analysis by  ...[more]

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