Transcriptomics

Dataset Information

296

S54 -dependent response to nitrogen limitation and virulence in Burkholderia cenocepacia H111


ABSTRACT: Members of the genus Burkholderia are versatile bacteria capable of colonizing highly diverse environmental niches. In this study, we investigated the global response of the opportunistic pathogen Burkholderia cenocepacia H111 to nitrogen limitation at the transcript and protein expression level. In addition to a classical response to nitrogen starvation, including the activation of glutamine synthetase, PII proteins and the two component regulatory system ntrBC, B. cenocepacia H111 also up-regulated polyhydroxybutyrate (PHB) accumulation and exopolysaccharide (EPS) production in response to nitrogen shortage. A search for consensus sequences in promoter regions of nitrogen responsive genes identified a s54 consensus sequence. The mapping of the s54 regulon as well as the characterization of a s54 mutant suggests an important role of s54 not only in control of nitrogen metabolism, but also in virulence of this organism. Nitrogen limitation and s54 regulon in B. cenocepacia

ORGANISM(S): Burkholderia cenocepacia H111  

SUBMITTER: Gerardo Cárcamo-Oyarce   Gabriella Pessi  Christian H Ahrens  Claudio Aguilar  Martina Lardi  Leo Eberl  Nadine Schmid  Ulrich Omasits  Alessandro Pedrioli  Angela Suppiger 

PROVIDER: E-GEOD-66328 | ArrayExpress | 2015-04-29

SECONDARY ACCESSION(S): SRP055546GSE66328PRJNA276555

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

σ54-Dependent Response to Nitrogen Limitation and Virulence in Burkholderia cenocepacia Strain H111.

Lardi Martina M   Aguilar Claudio C   Pedrioli Alessandro A   Omasits Ulrich U   Suppiger Angela A   Cárcamo-Oyarce Gerardo G   Schmid Nadine N   Ahrens Christian H CH   Eberl Leo L   Pessi Gabriella G  

Applied and environmental microbiology 20150403 12


Members of the genus Burkholderia are versatile bacteria capable of colonizing highly diverse environmental niches. In this study, we investigated the global response of the opportunistic pathogen Burkholderia cenocepacia H111 to nitrogen limitation at the transcript and protein expression levels. In addition to a classical response to nitrogen starvation, including the activation of glutamine synthetase, PII proteins, and the two-component regulatory system NtrBC, B. cenocepacia H111 also upreg  ...[more]

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