Transcriptomics

Dataset Information

296

Differential RNA-seq of Plant Beneficial Rhizobacterium Bacillus amyloliquefaciens FZB42 Reveals sRNA Bas01 involved in Sporulation and Biofilm Formation


ABSTRACT: Bacillus amyloliquefaciens FZB42 is a representative organism for Gram positive soil bacteria associated with plant roots and beneficial to plant growth. It is of immense importance to understand mechanisms of this class of bacteria adapting to rhizosphere. In this work employing differential RNA sequencing (RNA-seq) and Northern blot, we systematically identified transcription start sites of mRNAs as well as non-coding regulatory RNAs in FZB42. The genes regulated at different growth phases and located in polycistronic operons were also identified. A set of genes were re-annotated. In addition, a sRNA named Bas01 was identified to be involved in Bacillus sporulation and biofilm formation. The result we obtained provides valuable data for investigation of Bacillus gene expression and molecular details of rhizobacterial interaction with host plants. Examination of transcriptome profile of rhizobacterium B. amyloliquefaciens FZB42 grown under six conditions.

ORGANISM(S): Bacillus velezensis FZB42  

SUBMITTER: Lei Li   Yanjie Chao  Rainer Borriss  Jörg Vogel  Ben Fan  Xiao Q Wu  Konrad Förstner 

PROVIDER: E-GEOD-66681 | ArrayExpress | 2015-10-28

SECONDARY ACCESSION(S): SRP055994GSE66681PRJNA277720

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

dRNA-Seq Reveals Genomewide TSSs and Noncoding RNAs of Plant Beneficial Rhizobacterium Bacillus amyloliquefaciens FZB42.

Fan Ben B   Li Lei L   Chao Yanjie Y   Förstner Konrad K   Vogel Jörg J   Borriss Rainer R   Wu Xiao-Qin XQ  

PloS one 20151105 11


Bacillus amyloliquefaciens subsp. plantarum FZB42 is a representative of Gram-positive plant-growth-promoting rhizobacteria (PGPR) that inhabit plant root environments. In order to better understand the molecular mechanisms of bacteria-plant symbiosis, we have systematically analyzed the primary transcriptome of strain FZB42 grown under rhizosphere-mimicking conditions using differential RNA sequencing (dRNA-seq). Our analysis revealed 4,877 transcription start sites for protein-coding genes, id  ...[more]

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