Transcriptomics

Dataset Information

50

Gene expression profiling of B lymphocytes and plasma cells from Waldenstrons macroglobulinemia.


ABSTRACT: The tumoral clone of Waldenstrons macroglobulinemia (WM) shows a wide morphological heterogeneity which ranges from B-lymphocytes (BL) to plasma cells (PC). By means of genome-wide expression profiling we have been able to identify genes exclusively deregulated in BL and PC from WM, but with a similar expression pattern in their corresponding cell-counterparts from CLL and MM, as well as normal individuals. The differentially expressed genes have important functions in B-cell differentiation and oncogenesis. Thus, two of the genes down-regulated in WM-BL were IL4R, which plays a relevant role in CLL B cell survival, and BACH2 that participates in the development of class-switched PC. Interestingly, one of the up-regulated genes in WM-BL was IL6. A set of 4 genes was able to discriminate clonal B-lymphocytes from WM and CLL: LEF1 (WNT/ßcatenin pathway), MARCKS, ATXN1 and FMOD. We also found deregulation of genes involved in plasma cell differentiation such as PAX5 which was overexpressed in WM-PC, and IRF4 and BLIMP1 which were underexpressed. In addition, three of the target genes activated by PAX5 -CD79, BLNK and SYK- were up-regulated in WM-PC. In summary, these results indicate that both PC and BL from WM are genetically different from the MM and CLL cell-counterpart. Keywords: Waldenstrons macroglobulinemia, expression profiling, microarrays, Affymetrix. Bone marrow (BM) samples from 10 patients with Waldenstrons macroglobulinemia (WM), 12 with multiple myeloma (MM) and 11 with chronic lymphocytic leukemia (CLL) were included in the study. All samples corresponded to newly diagnosed untreated patients. In addition, 8 normal B lymphocytes samples (NBL) from peripheral blood and 5 normal plasma cells (NPC) from bone marrow of healthy donors were also selected in order to relate the deregulation of GEP of clonal populations to normal condition. The study was approved by the local research ethics committee and written informed consent was obtained from all patients and healthy donors.

REANALYSED by: E-GEOD-6691

ORGANISM(S): Homo sapiens  

SUBMITTER: M J Arcos   J M Hernandez  M Delgado  M L Sanchez  J De las Rivas  N Carmen Gutierrez  P Maiso  J F San Miguel  E Ferminan  E M Ocio 

PROVIDER: E-GEOD-6691 | ArrayExpress | 2007-03-01

SECONDARY ACCESSION(S): GSE6691PRJNA99073

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

Gene expression profiling of B lymphocytes and plasma cells from Waldenström's macroglobulinemia: comparison with expression patterns of the same cell counterparts from chronic lymphocytic leukemia, multiple myeloma and normal individuals.

Gutiérrez NC   Ocio EM   de Las Rivas J   Maiso P   Delgado M   Fermiñán E   Arcos MJ   Sánchez ML   Hernández JM   San Miguel JF  

Leukemia 20070125 3


The tumoral clone of Waldenström's macroglobulinemia (WM) shows a wide morphological heterogeneity, which ranges from B lymphocytes (BL) to plasma cells (PC). By means of genome-wide expression profiling we have been able to identify genes exclusively deregulated in BL and PC from WM, but with a similar expression pattern in their corresponding cell counterparts from chronic lymphocytic leukemia (CLL) and multiple myeloma (MM), as well as normal individuals. The differentially expressed genes ha  ...[more]

Similar Datasets

2014-08-04 | E-GEOD-6691 | ExpressionAtlas
| GSE9656 | GEO
2008-12-05 | E-GEOD-9656 | ArrayExpress
2012-07-09 | E-GEOD-39189 | ArrayExpress
2015-05-05 | E-GEOD-61597 | ArrayExpress
2010-05-20 | E-GEOD-15145 | ArrayExpress
2007-08-23 | E-GEOD-8472 | ArrayExpress
| GSE12668 | GEO
2007-08-23 | GSE8472 | GEO
2009-04-27 | GSE15145 | GEO