Proteomics

Dataset Information

3

Reverse phase protein arrays (RPPAs): effects of MPS1 inhibition on signaling pathways in GBM cells


ABSTRACT: To determine the biological effects of MPS1 inhibition (both by siRNA and Drug (NMSP715)) on signaling pathways in GBM cells (U251 &U87), we profiled the modulation of phosphorylated and non-phosphorylated proteins using RPPA Relative protein levels for each sample were determined by interpolation of each dilution curves from the standard curve antibody slide. All the data points were normalized for protein loading and transformed to a linear value. Linear values were transformed to Log2 value and then median‐centered for hierarchical cluster analysis.

ORGANISM(S): Homo sapiens  

SUBMITTER: Uma T Shankavaram   Kevin Camphausen  Uday B Maachani 

PROVIDER: E-GEOD-67502 | ArrayExpress | 2015-06-25

SECONDARY ACCESSION(S): GSE67502PRJNA280091

REPOSITORIES: GEO, ArrayExpress

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Publications

Modulation of miR-21 signaling by MPS1 in human glioblastoma.

Maachani Uday B UB   Tandle Anita A   Shankavaram Uma U   Kramp Tamalee T   Camphausen Kevin K  

Oncotarget 20160801 33


Monopolar spindle 1 (MPS1) is an essential spindle assembly checkpoint (SAC) kinase involved in determining spindle integrity. Beyond its mitotic functions, it has been implicated in several other signaling pathways. Our earlier studies have elaborated on role of MPS1 in glioblastoma (GBM) radiosensitization. In this study using reverse phase protein arrays (RPPAs), we assessed MPS1 mediated cell signaling pathways and demonstrated that inhibiting MPS1 could upregulate the expression of the tumo  ...[more]

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