Project description:HCC cell line SMMC-7721 were treatment with human recombinant artemin for 12 hours. Total RNA was extracted and the induced gene expression was analyzed. Analysis of gene expression induced by artemin treatment
Project description:In our present study, we found that lncRNA-hPVT1 could promote HCC cell proliferation. We want to know what were the target genes of lncRNA-hPVT1. So we constructed the hPVT1-overexpressed SMMC-771 cells and observed the mRNA profile in hPVT1-overexpressed and control SMMC-771 cells. lncRNA-hPVT1 induced transcriptional changes in SMMC-7721. Two-condition experiment, control (LV-Control) vs lncRNA-hPVT1 expression (LV-hPVT1 Clone2 ). Each has 3 biological repeats.
Project description:To gain insights into the mechanisms of Eupolyphaga sinensis Walker (ESW) and taspine derivate on inhibition to HCC, we have employed whole genome microarray expression profiling as a discovery platform to identify different genes between ESW-treated sample and control. At the same time, the differences were investigated between taspine derivate and the same control. SMMC-7721 cells were cultivated in the absence or presence of 0.1mg/mL ESWE and Taspine derivate of 2.5x10-5mol/L for 48 h, followed by the Agilent Whole Human Genome Oligo Microarray. The PLG, PKCβ and IL3RA genes confirmation of Microarray analysis was confirmed by Real-time PCR. SMMC-7721 cells were cultivated in the absence or presence of 0.1mg/mL Eupolyphaga sinensis Walker extract (ESWE) and Taspine derivate of 2.5x10-5mol/L for 48 h, followed by the Agilent Whole Human Genome Oligo Microarray
Project description:The role of TGF-β-induced epithelial-mesenchymal transition (EMT) in cancer cell dissemination is well established, but the involvement of lncRNAs in TGF-β signaling is still unknown. In this study, we observed that the lncRNA-Activated by TGF-β (lncRNA-ATB) was upregulated in hepatocellular carcinoma (HCC) metastases and associated with poor prognosis. lncRNA-ATB promotes the invasion-metastasis cascade, which suggest that lncRNA-ATB, a mediator of TGF-β signaling, could predispose HCC patients to metastases and may serve as a potential target for anti-metastatic therapies. SMMC-7721 hepatoma cells were continuously treated with 10 ng/ml of recombinant TGF-β1 for 21 days. Total RNA recovered from three untreated cells and three treated cells were used to acquire different expression profiles of mRNAs and lncRNAs.
Project description:We performed a genome wide transcription profile analysis to determine the expression alterations between control and the POH1 siRNAs transfected SMMC-7721 cells The liver cancer cell line SMMC-7721 transfected with either the control or POH1 siRNAs were subjected to a genome wide transcription profile analysis through Human U133 Puls 2.0 (Affymetrix) microarray
Project description:The goals of this study are to analyze the transcriptome profiling (RNA-seq) after PBX3 overexpression in SMMC-7721 cells. Overall design: SMMC-7721 cells were transfected by PBX3 expression lentivirus and blank lentivirus as control. Total RNA was extraced by Qiagen kit and the mRNA profiles were generated by deep sequencing, in duplicate, using Illumina HiSeq 2000.
Project description:We report the high-throughput profiling of PBX3-binding sites under liver cancer stem cell reprogramming driven by PBX3 overexpression. By obtaining over four billion bases of sequence from chromatin immunoprecipitated DNA, we generated genome-wide PBX3-binding maps of SMMC-7721 cells. This study provides a prediction of regulated genes by the PBX3. Overall design: Examination of PBX3-binding sites in SMMC-7721 cell with overexpression PBX3. The fragmented DNA was precipitated with a PBX3 antibody.
Project description:Our study have demonstrated LINC01138 acts as an oncogenic driver that promotes cell proliferation, tumourigenicity, tumour invasion and metastasis through physically interacting with Arginine Methyltransferase 5 (PRMT5) in HCC cells. In order to investigate the related signaling pathways regulated by LINC01138 or PRMT5, we performed the unbiased transcriptome profiling using high-throughput RNA sequencing in SMMC-7721 cells transfected with si-LINC01138 or si-PRMT5. Here we showed the LINC01138/PRMT5 axis is an ideal therapeutic target for HCC treatment. Overall design: Illuminate the related signaling pathways and biological processes regulated by LINC01138 or PRMT5 in human liver cancer SMMC-7721 cells through high-throughput sequencing data.