Transcriptomics

Dataset Information

92

RNAseq and Lariat sequencing of different S. pombe growth conditions


ABSTRACT: We performed lariat sequencing to profile the diversity of spliced RNA lariats in S. pombe identify annotated and alternate introns. Three different growth conditions were used to grow S. pombe wt and S. pombe Δdbr1. Lariat sequencing of the Δdbr1 strains and RNAseq of the wt and Δdbr1 strains were done to profile intron lariats and exon-exon junctions in RNA transcripts.

ORGANISM(S): Schizosaccharomyces pombe  

SUBMITTER: Elizabeth Fogarty  Andrew Grimson   Jeffrey Pleiss   Jeffrey A. Pleiss   Nick Stepankiw    

PROVIDER: E-GEOD-68345 | ArrayExpress| 2015-09-04

SECONDARY ACCESSION(S): GSE68345SRP057746PRJNA282490

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Widespread alternative and aberrant splicing revealed by lariat sequencing.

Stepankiw Nicholas N   Raghavan Madhura M   Fogarty Elizabeth A EA   Grimson Andrew A   Pleiss Jeffrey A JA  

Nucleic acids research 20150810 17


Alternative splicing is an important and ancient feature of eukaryotic gene structure, the existence of which has likely facilitated eukaryotic proteome expansions. Here, we have used intron lariat sequencing to generate a comprehensive profile of splicing events in Schizosaccharomyces pombe, amongst the simplest organisms that possess mammalian-like splice site degeneracy. We reveal an unprecedented level of alternative splicing, including alternative splice site selection for over half of all  ...[more]

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