Transcriptomics,Multiomics

Dataset Information

2

Yeast m6A methylated mRNAs are enriched on translating ribosomes during meiosis, and under Rapamycin treatment


ABSTRACT: Interest in mRNA methylation has exploded in recent years. The sudden interest in a 40 year old discovery was due in part to the recent finding of FTO?s (Fat Mass Obesity) N6-methyl-adenosine (m6A) deaminase activity, thus suggested a link between obesity-associated diseases and the presence of m6A in mRNA. Another catalyst of the sudden rise in mRNA methylation research was the release of mRNA methylomes for human, mouse and Saccharomyces cerevisiae. However, the molecular function, or functions of this mRNA ?epimark? remain to be discovered. There is supportive evidence that m6A could be a mark for mRNA degradation due to its binding to YTH domain proteins, and consequently being chaperoned to P bodies. Nonetheless, only a subpopulation of the methylome was found binding to YTHDF2 in HeLa cells. The model organism Saccharomyces cerevisiae, has only one YTH domain protein (Pho92, Mrb1), which targets PHO4 transcripts for degradation under phosphate starvation. However, mRNA methylation is only found under meiosis inducing conditions, and PHO4 transcripts are apparently non-methylated. In this paper we set out to investigate if m6A could function alternatively to being a degradation mark in S. cerevisiae, we also sought to test whether it can be induced under non-standard sporulation conditions. We find associations between the presence of m6A and message translatability. We also find m6A induction following prolonged rapamycin treatment. GeneChip analyses were performed to investigate the distribution of early meiotic transcripts (3hours) on different polysome and monosome fractions

OTHER RELATED OMICS DATASETS IN: PRJNA282733

ORGANISM(S): Saccharomyces cerevisiae SK1  

SUBMITTER: Sean T May   Rupert G Fray  Andrew Bottley  Nathan Archer  Zsuzsanna Bodi  Eva Haeffner 

PROVIDER: E-GEOD-68435 | ArrayExpress | 2015-08-12

SECONDARY ACCESSION(S): GSE68435PRJNA282733

REPOSITORIES: GEO, ArrayExpress

Dataset's files

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Action DRS
E-GEOD-68435.idf.txt Idf
E-GEOD-68435.processed.1.zip Processed
E-GEOD-68435.raw.1.zip Raw
E-GEOD-68435.sdrf.txt Txt
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Publications

Yeast m6A Methylated mRNAs Are Enriched on Translating Ribosomes during Meiosis, and under Rapamycin Treatment.

Bodi Zsuzsanna Z   Bottley Andrew A   Archer Nathan N   May Sean T ST   Fray Rupert G RG  

PloS one 20150717 7


Interest in mRNA methylation has exploded in recent years. The sudden interest in a 40 year old discovery was due in part to the finding of FTO's (Fat Mass Obesity) N6-methyl-adenosine (m6A) deaminase activity, thus suggesting a link between obesity-associated diseases and the presence of m6A in mRNA. Another catalyst of the sudden rise in mRNA methylation research was the release of mRNA methylomes for human, mouse and Saccharomyces cerevisiae. However, the molecular function, or functions of t  ...[more]

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