Transcriptomics

Dataset Information

3

Functionally distinct subsets of lineage-biased multipotent progenitors control blood production in normal and regenerative conditions


ABSTRACT: To identify the molecular characterisitics of parallel lineage-biased MPP populations arising from hematopoietic stem cells (HSC) we conducted genome-wide analyses of hematopoietic stem, progenitor and mature myeloid cell populations using Affymetrix Gene ST1.0 arrays. Microarray analysis of 3-5 biological replicates of the indicated hematopoietic populations, isolated by FACS sorting from C57BL/6 mouse BM. Immunophenotypic definitions: Long-term HSC (HSCLT) (Lin-/cKit+/Sca1+/Flk2-/CD48-/CD150+); Short-term HSC (HSCST) (Lin-/cKit+/Sca1+/Flk2-/CD48-/CD150-); MPP2 (Lin-/cKit+/Sca1+/Flk2-/CD48+/CD150+); MPP3 (Lin-/cKit+/Sca1+/Flk2-/CD48+/CD150-); MPP4 (Lin-/cKit+/Sca1+/Flk2+); CMP (Lin-/cKit+/FcγR-/CD34+); GMP (Lin-/cKit+/FcγR+/CD34+); Pre-granulocyte (PreGr) (Mac1+/Gr1int); Granulocyte (Gr) (Mac1+/Gr1hi). HSC and GMP samples listed here were also used as controls for our related microarray study GSE48893.

ORGANISM(S): Mus musculus  

SUBMITTER: Eric M Pietras   Emmanuelle Passegué  Eric Martin Pietras 

PROVIDER: E-GEOD-68529 | ArrayExpress | 2015-05-05

SECONDARY ACCESSION(S): GSE68529PRJNA282994

REPOSITORIES: GEO, ArrayExpress

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