Transcriptomics

Dataset Information

3

The regenerative response in the pig liver remnant varies with the degree of resection and rise in portal pressure.


ABSTRACT: Following parenchymal loss, the liver regenerates restoring normal mass and metabolic function. Prevailing theories on triggering events leading to regeneration include humoral, metabolic and flow-mediated mechanisms, the latter emphasizing the importance of shear stress mediated nitric oxide (NO) regulation. We aimed to investigate whether the grade of resection and hence the portal venous pressure and sinusoidal shear stress increase, would be reflected in the gene expression profiles in the liver remnant by employing a global porcine cDNA microarray chip with approximately 23 000 genes represented. Six pig livers were resected with 62% (Low Portal Pressure Resection, LPPR) and 75% (High Portal Pressure Resection) resulting in a portal venous pressure increase from a baseline of 6.1 mmHg to 8.2 and 12 mmHg respectively. By sampling consecutive biopsies from the liver remnants we found differentially expressed genes in the HPPR group to have functions related primarily to apoptosis, nitric oxide metabolism and oxidative stress, whereas differentially expressed genes in the LPPR group potentially regulate the cell cycle. Common to both groups was the upregulation of genes regulating inflammation, transport, cell proliferation and development and protein metabolism. Also common to both groups was both up- and downregulation of genes regulating cell-cell signaling, signal transduction, cell adhesion and translation. Genes regulating the metabolism of lipids, hormones, amines, and alcohol were downregulated in both groups. Conclusions: The genetic regenerative response in the liver remnant to varies according to the level of resection. Keywords: time course, treatment comparison Three pigs underwent a 62% liver resection (low-pressure resection, LPR) and three underwent a 75% resection (high-pressure resection, HPR). Biopsies from the liver remnant were taken from all animals at time points 1, 30, 90, minutes and 3, 4 and 6 hours after resection. Expression profiling was conducted by hybridising each sample against a common reference, consisting of liver RNA from an unrelated animal.

ORGANISM(S): Sus scrofa  

SUBMITTER: Kim E Mortensen   Jakob Hedegaard  Lene N Conley  Trine Kalstad  Christian Bendixen  Arthur Revhaug  Peter Sørensen 

PROVIDER: E-GEOD-6860 | ArrayExpress | 2008-01-14

SECONDARY ACCESSION(S): GSE6860PRJNA99105

REPOSITORIES: GEO, ArrayExpress

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Regenerative response in the pig liver remnant varies with the degree of resection and rise in portal pressure.

Mortensen Kim Erlend KE   Conley Lene Nagstrup LN   Hedegaard Jakob J   Kalstad Trine T   Sorensen Peter P   Bendixen Christian C   Revhaug Arthur A  

American journal of physiology. Gastrointestinal and liver physiology 20080110 3


After parenchymal loss, the liver regenerates restoring normal mass and metabolic function. Prevailing theories on triggering events leading to regeneration include humoral, metabolic, and flow-mediated mechanisms, the latter emphasizing the importance of shear stress mediated nitric oxide regulation. We aimed to investigate whether the grade of resection and hence the portal venous pressure and sinusoidal shear stress increase would be reflected in the gene expression profiles in the liver remn  ...[more]

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