Transcriptomics

Dataset Information

36

Human Calu-3 cell micro RNA transcriptome response to a wild type infectious clone of H7N9 Influenza virus and mutant H7N9 viruses


ABSTRACT: The purpose is to obtain samples for mRNA, miRNA, proteomics, lipidomics, metabolomics, and histopathology analysis in human Calu-3 cells infected with WT A/Anhui/1/2013 (H7N9; 'AH1'), and AH - NS1-103F/106M. Human Calu-3 cells were seeded at 1 x 10^6 cells per well on 6 well plates 2 days before infection. Prior to infection, washed cells 2 times with PBS, and then infected with a multiplicity of infection of 1. Infected samples were collected in quintuplet; time-matched mocks were collected in quintuplet in parallel with infected samples. Time points: 7 and 12 h post-infection

ORGANISM(S): Homo sapiens  

SUBMITTER: Amie J Eisfeld  Kelly Stratton   Katrina M Waters   Yoshihiro Kawaoka   Kevin Walters    

PROVIDER: E-GEOD-69027 | ArrayExpress| 2015-05-23

SECONDARY ACCESSION(S): GSE69027PRJNA284341

REPOSITORIES: GEO, ArrayExpress

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