Transcriptomics

Dataset Information

63

Transcription profiling of E.coli BW25113 ymgB and W/T strains LBglu 24h biofilm cells


ABSTRACT: For the microarray experiments, 10 g glass wool (Corning Glass Works, Corning, N.Y.) were used to form biofilms (30) in 250 mL in 1 L Erlenmeyer shake flasks which were inoculated with overnight cultures diluted that were 1:167. The cells were shaken at 250 rpm and 37°C for 24 hours to form biofilms on the glass wool, and RNA was isolated from the suspension cells and the biofilm. Experiment Overall Design: Strain: E. coli BW25113 wild type and ymgB mutant Experiment Overall Design: Medium: LBglu Experiment Overall Design: Cells: biofilm cells on glass wool Experiment Overall Design: Time: 24h

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Escherichia coli  

SUBMITTER: Jintae Lee  

PROVIDER: E-GEOD-6925 | ArrayExpress | 2008-06-15

SECONDARY ACCESSION(S): GSE6925PRJNA99349

REPOSITORIES: GEO, ArrayExpress

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Publications

Structure and function of the Escherichia coli protein YmgB: a protein critical for biofilm formation and acid-resistance.

Lee Jintae J   Page Rebecca R   García-Contreras Rodolfo R   Palermino Jeanne-Marie JM   Zhang Xue-Song XS   Doshi Ojus O   Wood Thomas K TK   Peti Wolfgang W  

Journal of molecular biology 20070802 1


The Escherichia coli gene cluster ymgABC was identified in transcriptome studies to have a role in biofilm development and stability. In this study, we showed that YmgB represses biofilm formation in rich medium containing glucose, decreases cellular motility, and protects the cell from acid indicating that YmgB has a major role in acid-resistance in E. coli. Our data show that these phenotypes are potentially mediated through interactions with the important cell signal indole. In addition, gel  ...[more]

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