Transcriptomics

Dataset Information

5

Transcription profiling of mouse developing kidney to investigate Wnt4-dependent gene expression (GUDMAP Series_id: 13)


ABSTRACT: Our laboratory's interest is in understanding the molecular principles that underlie the regional organization of the mammalian metanephric kidney. Our goal is to generate a detailed spatial map of the cellular expression of selected regulatory genes during mammalian kidney development. The goal of this study is to identify a population of genes that are enriched in the renal vesicle (RV) and its derivatives using Wnt4 mutants. Experiment Overall Design: Wnt4 is required for renal vesicle (RV) induction. Therefore, RV and the derivatives (s-shaped body and eventually the mature nephron) are missing in Wnt4 mutants. At E14.5, these structures are present in wildtype kidneys. Transcriptional profile comparison between E14.5 wildtype and Wnt4 mutants therefore identify genes expressed in the RV and derivatives. Minimally pooled kidney samples were used as single biological replicates. Total RNA was isolated from these pools and subjected to a single round of amplification for use on Affymetrix arrays.

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Mus musculus  

SUBMITTER: Mehran Sharghi  

PROVIDER: E-GEOD-6934 | ArrayExpress | 2008-06-15

SECONDARY ACCESSION(S): GSE6934PRJNA98109

REPOSITORIES: GEO, ArrayExpress

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