Comparison of endogenous tRNA expression levels from cells expressing and sgRNA from either a U6 or tRNA promoter
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ABSTRACT: The goal of this study was to compare the endogenous tRNA expression levels from cells transduced with a lentiviral vector encopding Cas9 and an sgRNA from a U6 or a Glutamine tRNA promoter. Using high-throughput sequencing methods (Illumina Hi-Seq 2000) we show that endogenous tRNA expression levels are not perturbed by expression of an sgRNA from a human tRNA. tRNA expression profiles were generated from 2 different transduced polyconal cell lines.
ORGANISM(S): Homo sapiens
SUBMITTER:
Adam Mefferd Adam Lee Mefferd Bryan Cullen
PROVIDER: E-GEOD-69720 | ArrayExpress | 2015-09-09
SECONDARY ACCESSION(S): GSE69720SRP059359PRJNA286739
REPOSITORIES: GEO, ArrayExpress, ENA
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