Genomics,Multiomics

Dataset Information

2

Cane Toad Metamorphis


ABSTRACT: Comparison of pre and post metamorphis phases of cane toad Keywords: Developmental Stages Samples were harvested at 9, 18, 28 (pre-metamorph), 30 and 53 (post-metamorph) days of age and RNA extracted using Trizol Reagent (Invitrogen). Total RNA (100 mg) was reverse transcribed (Qiagen) and labelled cDNA probes were generated using the fluorophores, Cy3 and Cy5 (Amersham Pharmacia Biotech). Prehybridization of slides, application of the probe to the microarray slides, hybridisation, and subsequent washing steps were performed according to manufacturer's instructions (Corning Microarray Technology). Slides were scanned and analysed with a GenePix 4000B laser scanner and GenePix 3.0 and 4.0 software (Axon Instruments).

OTHER RELATED OMICS DATASETS IN: PRJNA99257

ORGANISM(S): Rhinella marina  

SUBMITTER: Gavin C Kennedy   Damien C Halliday  Andrew Spriggs 

PROVIDER: E-GEOD-6987 | ArrayExpress | 2008-11-27

SECONDARY ACCESSION(S): GSE6987PRJNA99257

REPOSITORIES: GEO, ArrayExpress

Dataset's files

Source:
Action DRS
E-GEOD-6987.README.txt Txt
E-GEOD-6987.eSet.r Other
E-GEOD-6987.idf.txt Idf
E-GEOD-6987.processed.1.zip Processed
E-GEOD-6987.raw.1.zip Raw
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Publications

Genes induced during the early developmental stages of the Cane Toad, Bufo (Chaunus) marinus.

Halliday Damien C T DCT   Kennedy Gavin C GC   Hamilton Nicholas H R NHR   Tarmo Suze S   Alderman James J   Siddon Nicole A NA   Robinson Anthony J AJ  

Gene expression patterns : GEP 20080426 6


Metamorphosis, a critical stage in the development of toads and frogs, involves rapid levels of morphological change. In the current study, we have used microarray analysis to identify shifts in gene expression between tadpole and toadlet stages of the cane toad, Bufo (Chaunus) marinus. Here, we report on nine genes that show the greatest induction during metamorphosis; the gut-associated gastrokine and trefoil factor, blood components haemoglobins alpha/beta, apolipoprotein and serum albumin, a  ...[more]

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