Transcriptomics

Dataset Information

37

Difference of gene expression between LPS-stimulated monocytes cultured in the presence NRBCs and those cultured in the absence of NRBCs


ABSTRACT: We found that human cord blood nucleated red blood cells (NRBCs) have a regulatory function in the innate immune reaction. NRBCs suppressed the production of inflammatory cytokines including TNF-a and IL-1b from monocytes in response to lipopolysaccharide (LPS). NRBCs exerted the regulatory function even without cell-to-cell contact with monocytes. On the other hand, IL-10 production from monocytes by the stimulation of LPS in the presence of NRBCs was higher than that of LPS-stimulated monocytes cultured in the absence of NRBCs. Addition of an anti-IL-10 receptor blocking antibody restored the inflammatory cytokine production from monocytes, suggesting that the functional change of monocytes caused by the interaction of NRBCs was characterized and mediated by the increased IL-10 production. Whole-genome microarray analysis revealed that monocytes expressed increased amounts of IL-10 superfamily genes after the interaction with NRBCs. IL-19, one of the IL-10 superfamily, enhanced IL-10 production from monocytes, which suggested cooperative role of IL-10 superfamily in the suppression of inflammatory cytokine production from monocytes. Arginase which was reported to play an important role in the suppressive function of NRBCs on monocytes in mice was found to play no significant role in human monocytes. NRBCs seem to have a regulatory role to suppress vigorous innate immune reaction which can be harmful to the fetuses via some unknown soluble factor which enhances the production of IL-10 and IL-10 family cytokines in monocytes. Human LPS-stimulated monocytes were collected after culture in a condition without and with NRBCs in transwell culture system. Only one experiment was performed.

ORGANISM(S): Homo sapiens  

SUBMITTER: Hidetoshi Takada  Lili Cui   Cui Li Li    

PROVIDER: E-GEOD-70146 | ArrayExpress | 2016-01-18

SECONDARY ACCESSION(S): GSE70146PRJNA287783

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

Sorry, this publication's infomation has not been loaded in the Indexer, please go directly to PUBMED or Altmetric.

Similar Datasets

2014-05-02 | E-GEOD-6002 | ArrayExpress
2008-09-30 | GSE6002 | GEO
| GSE102135 | GEO
2016-04-04 | E-GEOD-79631 | ArrayExpress
| GSE79631 | GEO
| GSE101617 | GEO
2006-07-04 | GSE5220 | GEO
2012-03-27 | E-GEOD-9509 | ArrayExpress
2007-12-01 | GSE9509 | GEO
2008-03-20 | GSE9988 | GEO