Transcriptomics

Dataset Information

4

The NuRD chromatin remodelling complex regulates brain cortical development


ABSTRACT: Dynamic changes of histone epigenetic modifications and chromatin structure represent an universal mechanism by which cells adapt their transcriptional response to rapidly changing environmental conditions. During neuronal development, extensive chromatin remodeling takes place allowing the transition of pluripotent cells into differentiated neurons. Here we report that the ATP-dependent chromatin remodeling complex NuRD, which couples ATP-dependent nucleosome sliding with histone deacetylase activity, is a major remodeling complex in embryonic brain and plays an instructive role during mouse neuronal development. Importantly, the ATPase subunits of NuRD complex CHD3, CHD4 and CHD5 undergo a functional switch, thereby regulating distinct aspects of neuronal differentiation and migration in a sequential and mostly non-overlapping manner. We conclude that the recruitment of NuRD complexes containing specific CHDs to gene promoters and enhancers plays an instructive role in brain development. Gene expression analysis was performed in the mouse embryonic cortex at three developmental stages: E12.5, E15.5 and E18.5 using total RNA obtained from four embryos for each time point.

ORGANISM(S): Mus musculus  

SUBMITTER: Richard Mitter   Justyna Nitarska  Antonella Riccio 

PROVIDER: E-GEOD-70298 | ArrayExpress | 2015-06-27

SECONDARY ACCESSION(S): GSE70298PRJNA288281

REPOSITORIES: GEO, ArrayExpress

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