Dataset Information


Localization of Scc2 between wild type and scc4-m35 cells in S-phase

ABSTRACT: Wild-type (AM15307) and scc4-m35 (AM15311) cells carrying SCC2-3FLAG were fixed for 2 hr, 15 min following an a-factor arrest. Samples were harvested, anti-HA ChIP was performed and both input and IP samples were sequenced for both strains. ChIP-seq for S. cerevisiae Scc2-3FLAG in S-phase cells in Wild type and in scc4-m35 mutant Vasso Makrantoni: experiments and Alastair Kerr: ChIP Seq Data analysis

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Vasso Makrantoni   Alastair Kerr  Alastair Robert Kerr 

PROVIDER: E-GEOD-70316 | ArrayExpress | 2016-07-21



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The SNF2 family ATPase LSH promotes cell-autonomous de novo DNA methylation in somatic cells.

Termanis Ausma A   Torrea Natalia N   Culley Jayne J   Kerr Alastair A   Ramsahoye Bernard B   Stancheva Irina I  

Nucleic acids research 20160513 16

Methylation of DNA at carbon 5 of cytosine is essential for mammalian development and implicated in transcriptional repression of genes and transposons. New patterns of DNA methylation characteristic of lineage-committed cells are established at the exit from pluripotency by de novo DNA methyltransferases enzymes, DNMT3A and DNMT3B, which are regulated by developmental signaling and require access to chromatin-organized DNA. Whether or not the capacity for de novo DNA methylation of developmenta  ...[more]

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