Transcriptomics

Dataset Information

18

Transcription profiling of mouse brown and white adipocyte differentiated and undifferentiated states


ABSTRACT: Attainment of a brown adipocyte cell phenotype in white adipocytes, with their abundant mitochondria and increased energy expenditure potential, is a legitimate strategy for combating obesity. The unique transcriptional regulators of the primary brown adipocyte phenotype are unknown, limiting our ability to promote brown adipogenesis over white. In the present work, we used microarray analysis strategies to study primary preadipocytes, and we made the striking discovery that brown preadipocytes demonstrate a myogenic transcriptional signature, whereas both brown and white primary preadipocytes demonstrate signatures distinct from those found in immortalized adipogenic models. We found a plausible SIRT1-related transcriptional signature during brown adipocyte differentiation that may contribute to silencing the myogenic signature. In contrast to brown preadipocytes or skeletal muscle cells, white preadipocytes express Tcf21, a transcription factor that has been shown to suppress myogenesis and nuclear receptor activity. In addition, we identified a number of developmental genes that are differentially expressed between brown and white preadipocytes and that have recently been implicated in human obesity. The interlinkage between the myocyte and the brown preadipocyte confirms the distinct origin for brown versus white adipose tissue and also represents a plausible explanation as to why brown adipocytes ultimately specialize in lipid catabolism rather than storage, much like oxidative skeletal muscle tissue. Experiment Overall Design: Comparisons of white and brown pre- and mature-adiposytes

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Mus musculus  

SUBMITTER: Ola Larsson  

PROVIDER: E-GEOD-7032 | ArrayExpress | 2009-04-08

SECONDARY ACCESSION(S): GSE7032GDS2743PRJNA98391

REPOSITORIES: GEO, ArrayExpress

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Publications

Myogenic gene expression signature establishes that brown and white adipocytes originate from distinct cell lineages.

Timmons James A JA   Wennmalm Kristian K   Larsson Ola O   Walden Tomas B TB   Lassmann Timo T   Petrovic Natasa N   Hamilton D Lee DL   Gimeno Ruth E RE   Wahlestedt Claes C   Baar Keith K   Nedergaard Jan J   Cannon Barbara B  

Proceedings of the National Academy of Sciences of the United States of America 20070305 11


Attainment of a brown adipocyte cell phenotype in white adipocytes, with their abundant mitochondria and increased energy expenditure potential, is a legitimate strategy for combating obesity. The unique transcriptional regulators of the primary brown adipocyte phenotype are unknown, limiting our ability to promote brown adipogenesis over white. In the present work, we used microarray analysis strategies to study primary preadipocytes, and we made the striking discovery that brown preadipocytes  ...[more]

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