Transcriptomics

Dataset Information

301

TRNA-Derived Small Non-Coding RNAs in Response to Ischemia Inhibit Angiogenesis


ABSTRACT: Ischemic injuries will lead to necrotic tissue damage, and post-ischemia angiogenesis plays critical roles in blood flow restoration and tissue recovery. Recently, several types of small RNAs have been reported to be involved in this process. In this study, we first generated a rat brain ischemic model to investigate the involvement of new types of small RNAs in ischemia. We utilized deep sequencing and bioinformatics analyses to demonstrate that the level of small RNA fragments derived from tRNAs was strikingly up-regulated in the ischemic rat brain. Among these sequences, tRNAVal- and tRNAGly-derived small RNAs account for the most abundant segments. The up-regulation of tRNAVal- and tRNAGly-derived fragments was verified through northern blot and quantitative PCR analyses. The levels of these two fragments also increased in a mouse hindlimb ischemia model and cellular hypoxia model. Importantly, the overexpression of tRNAVal- and tRNAGly-derived fragments in endothelial cells inhibited cell proliferation, migration and tube formation. Our results indicate that tRNA-derived fragments are involved in tissue ischemia, and we demonstrate for the first time that tRNAVal- and tRNAGly-derived fragments inhibit angiogenesis by modulating the function of endothelial cells. Examine small RNAs involved in post-ischemia angiogenesis

ORGANISM(S): Rattus norvegicus  

SUBMITTER: Yang Wang   Qing Li 

PROVIDER: E-GEOD-70473 | ArrayExpress | 2016-03-16

SECONDARY ACCESSION(S): SRP060237GSE70473PRJNA288785

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

tRNA-Derived Small Non-Coding RNAs in Response to Ischemia Inhibit Angiogenesis.

Li Qing Q   Hu Bin B   Hu Guo-Wen GW   Chen Chun-Yuan CY   Niu Xin X   Liu Juan J   Zhou Shu-Min SM   Zhang Chang-Qing CQ   Wang Yang Y   Deng Zhi-Feng ZF  

Scientific reports 20160211


Ischemic injuries will lead to necrotic tissue damage, and post-ischemia angiogenesis plays critical roles in blood flow restoration and tissue recovery. Recently, several types of small RNAs have been reported to be involved in this process. In this study, we first generated a rat brain ischemic model to investigate the involvement of new types of small RNAs in ischemia. We utilized deep sequencing and bioinformatics analyses to demonstrate that the level of small RNA fragments derived from tRN  ...[more]

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