Proteomics

Dataset Information

5

Broad spectrum antibodies to self-antigens and cytokines in RAG deficiency


ABSTRACT: Overall, this work describes the largest cohort of patients with RAG mutations and an associated phenotype consisting of combined immunodeficiency and granulomatous lesions and/or autoimmunity (CID-G/AI). By using multiple methods (microarray, ELISA and multiplex bead technology), we have consistently identified a distinctive signature of anti-cytokine antibodies in patients with RAG-dependent immunodeficiencies, especially in those with CID-G/AI and a history of severe viral infections. These autoantibodies were not detected in a large panel of patients with other forms of primary immunodeficiency, and may therefore represent a novel biomarker panel of this condition. Known autoantigens, cytokines, chemokines, growth factors and receptors were printed onto microarrays. All targets were printed in triplicate. Arrays were probed with diluted plasma and autoantibodies were detected with a AF647 conjugated anti-human IgG secondary.

SUBMITTER: Jacob Rosenberg   Jolan Walter 

PROVIDER: E-GEOD-70618 | ArrayExpress | 2015-07-08

SECONDARY ACCESSION(S): GSE70618PRJNA289166

REPOSITORIES: GEO, ArrayExpress

Similar Datasets

2016-06-01 | E-GEOD-77949 | ArrayExpress
2013-09-24 | E-GEOD-51102 | ArrayExpress
| GSE62599 | GEO
2015-04-22 | E-GEOD-68118 | ArrayExpress
2015-04-22 | E-GEOD-68117 | ArrayExpress
2015-04-22 | E-GEOD-68116 | ArrayExpress
2015-04-22 | E-GEOD-68115 | ArrayExpress
2015-04-22 | E-GEOD-68114 | ArrayExpress
2013-09-24 | E-GEOD-51101 | ArrayExpress
2013-05-01 | E-GEOD-43446 | ArrayExpress