Transcriptomics

Dataset Information

3

Real-time quantitative PCR analysis for validation of miRNAs expressed during osteogenic differentiation of bone marrow MSCs versus healthy donors MSCs


ABSTRACT: Total small RNA was isolated from BM-MSCs obtained from OA diagnosed patients (n=10) and non-OA (n=10 after osteogenic induction for 1, 10 and 21 days. miRNAs, were initially screened and those with consistent differential expression were selected for further validation by qRT-PCR analysis. From an initial screening of 246 differentially expressed miRNAs (fold change ≥± 2, P ≤0.05) between OA and non-OA BM-MSC samples 21 were selected and validated by qRT-PCR

ORGANISM(S): Homo sapiens  

SUBMITTER: Lydia Abásolo   José Lamas  María Tomé  Enrique Moro  Eva Herranz  Benjamín Fernández-Gutiérrez  Pilar Tornero-Esteban  José Ramón Lamas Lamas  Pedro López-Romero  Manuel A González  Fernando Marco  Luis Rodríguez-Rodríguez 

PROVIDER: E-GEOD-70856 | ArrayExpress | 2015-07-14

SECONDARY ACCESSION(S): GSE70856PRJNA289693

REPOSITORIES: GEO, ArrayExpress

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