Real-time quantitative PCR analysis for validation of miRNAs expressed during osteogenic differentiation of bone marrow MSCs versus healthy donors MSCs
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ABSTRACT: Total small RNA was isolated from BM-MSCs obtained from OA diagnosed patients (n=10) and non-OA (n=10 after osteogenic induction for 1, 10 and 21 days. miRNAs, were initially screened and those with consistent differential expression were selected for further validation by qRT-PCR analysis. From an initial screening of 246 differentially expressed miRNAs (fold change ≥± 2, P ≤0.05) between OA and non-OA BM-MSC samples 21 were selected and validated by qRT-PCR
ORGANISM(S): Homo sapiens
SUBMITTER:
Lydia Abásolo José Lamas María Tomé Enrique Moro Eva Herranz Benjamín Fernández-Gutiérrez Pilar Tornero-Esteban José Ramón Lamas Lamas Pedro López-Romero Manuel A González Fernando Marco Luis Rodríguez-Rodríguez
PROVIDER: E-GEOD-70856 | ArrayExpress | 2015-07-14
SECONDARY ACCESSION(S): GSE70856PRJNA289693
REPOSITORIES: GEO, ArrayExpress
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