Dataset Information


Transcription profiling by array of human p53+ and p53- fibroblasts treated with arsenite

ABSTRACT: Microarray data from G2-synchronized p53(+) and p53(-) fibroblasts before and after 3 h release from cell cycle blockade in the presence of 5 uM sodium arsenite. Experiment Overall Design: Cells expressing p53 from a tet-off regulated construct were synchronized in G2 with a two-step procedure using 24 h aphidicolin treatment for initial G1 synchronization and 12 h of Hoechst 33342 to effect a G2 blockade. During Hoechst treatment, tetracycline was added to suppress p53 in half the cultures. Cells were then released into media containing 5 µM sodium arsenite and the appropriate concentration of tetracycline to maintaining p53 expression. mRNAs were collected at 0 h and 3 h after release from G2 synchrony.

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Homo sapiens  

SUBMITTER: Xiaoqiang Xu  

PROVIDER: E-GEOD-7101 | ArrayExpress | 2007-11-11



altmetric image


Exit from arsenite-induced mitotic arrest is p53 dependent.

McNeely Samuel C SC   Xu Xiaogiang X   Taylor B Frazier BF   Zacharias Wolfgang W   McCabe Michael J MJ   States J Christopher JC  

Environmental health perspectives 20060901 9

Arsenic is both a human carcinogen and a chemotherapeutic agent, but the mechanism of neither arsenic-induced carcinogenesis nor tumor selective cytotoxicity is clear. Using a model cell line in which p53 expression is regulated exogenously in a tetracycline-off system (TR9-7 cells) , our laboratory has shown that arsenite disrupts mitosis and that p53-deficient cells [p53(-)], in contrast to p53-expressing cells [p53(+)], display greater sensitivity to arsenite-induced mitotic arrest and apopto  ...[more]

Similar Datasets

2010-05-07 | E-GEOD-15547 | ArrayExpress
2016-06-26 | E-GEOD-79678 | ArrayExpress
2014-05-01 | E-GEOD-56178 | ArrayExpress
2010-05-07 | E-GEOD-15399 | ArrayExpress
2019-01-29 | PXD012463 | Pride
| PRJNA98441 | ENA
2016-07-03 | E-GEOD-72431 | ArrayExpress
2015-02-16 | E-GEOD-56253 | ArrayExpress
2015-03-22 | E-MTAB-3362 | ArrayExpress
2012-04-01 | E-MEXP-3526 | ArrayExpress