Dataset Information


Functional coordination and HuR-mediated regulation of mRNA stability during T cell activation

ABSTRACT: We evaluated changes in mRNA stability and transcription using 4sU metabolic pulse labeling across a four hour time course following activation of Jurkat T cells with PMA and PHA Measurement of total mRNA (T) and 4sU labeled mRNA (IP) in three biological replicates at five time points: prior to activation (U) and the first four hours after activation (1-4)

ORGANISM(S): Homo sapiens  

SUBMITTER: Jeff G Blackinton   Jack D Keene  Jeff Blackinton 

PROVIDER: E-GEOD-71382 | ArrayExpress | 2015-10-28



altmetric image


Functional coordination and HuR-mediated regulation of mRNA stability during T cell activation.

Blackinton Jeff G JG   Keene Jack D JD  

Nucleic acids research 20151020 1

Global mRNA abundance depends on the balance of synthesis and decay of a population of mRNAs. To account for this balance during activation of T cells, we used metabolic labeling to quantify the contributions of RNA transcription and decay over a 4 h time course during activation of leukemia-derived Jurkat T cells. While prior studies suggested more than half of the changes in mRNA abundance were due to RNA stability, we found a smaller but more interesting population of mRNAs changed stability.  ...[more]

Similar Datasets

2015-07-23 | E-GEOD-70481 | ArrayExpress
2010-09-01 | E-GEOD-23833 | ArrayExpress
2012-06-01 | E-GEOD-38356 | ArrayExpress
2014-04-11 | E-GEOD-56748 | ArrayExpress
2014-12-30 | E-MTAB-2691 | ArrayExpress
2019-04-05 | E-MTAB-7784 | ArrayExpress
2011-04-24 | E-GEOD-25432 | ArrayExpress
2014-03-01 | E-GEOD-54716 | ArrayExpress
2016-03-29 | E-GEOD-75220 | ArrayExpress
2013-01-08 | E-MTAB-1059 | ArrayExpress