Transcriptomics

Dataset Information

298

Functional coordination and HuR-mediated regulation of mRNA stability during T cell activation


ABSTRACT: We evaluated changes in mRNA stability and transcription using 4sU metabolic pulse labeling across a four hour time course following activation of Jurkat T cells with PMA and PHA Measurement of total mRNA (T) and 4sU labeled mRNA (IP) in three biological replicates at five time points: prior to activation (U) and the first four hours after activation (1-4)

ORGANISM(S): Homo sapiens  

SUBMITTER: Jeff G Blackinton   Jack D Keene  Jeff Blackinton 

PROVIDER: E-GEOD-71382 | ArrayExpress | 2015-10-28

SECONDARY ACCESSION(S): SRP061639GSE71382PRJNA291072

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Functional coordination and HuR-mediated regulation of mRNA stability during T cell activation.

Blackinton Jeff G JG   Keene Jack D JD  

Nucleic acids research 20151020 1


Global mRNA abundance depends on the balance of synthesis and decay of a population of mRNAs. To account for this balance during activation of T cells, we used metabolic labeling to quantify the contributions of RNA transcription and decay over a 4 h time course during activation of leukemia-derived Jurkat T cells. While prior studies suggested more than half of the changes in mRNA abundance were due to RNA stability, we found a smaller but more interesting population of mRNAs changed stability.  ...[more]

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