Transcriptomics

Dataset Information

297

In vivo transcriptional activation using CRISPR-Cas9 in Drosophila


ABSTRACT: Identifying putative transcription factor target genes by combining CRISPR/Cas9-based transcriptional activation with RNAseq in Drosophila S2R+ cells. This study focuses on the transcription factors Twist and Snail, singly and together. RNA from Drosophila cells following CRISPR/Cas9-based activation of Twist, Snail, or Twist and Snail together, compared with non-targeting sgRNA. Two biological replicates for each experiment

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: Nobert Perrimon   Norbert Perrimon  Xiaochun Ni 

PROVIDER: E-GEOD-71430 | ArrayExpress | 2015-08-10

SECONDARY ACCESSION(S): GSE71430SRP061691PRJNA291173

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

In Vivo Transcriptional Activation Using CRISPR/Cas9 in Drosophila.

Lin Shuailiang S   Ewen-Campen Ben B   Ni Xiaochun X   Housden Benjamin E BE   Perrimon Norbert N  

Genetics 20150805 2


A number of approaches for Cas9-mediated transcriptional activation have recently been developed, allowing target genes to be overexpressed from their endogenous genomic loci. However, these approaches have thus far been limited to cell culture, and this technique has not been demonstrated in vivo in any animal. The technique involving the fewest separate components, and therefore the most amenable to in vivo applications, is the dCas9-VPR system, where a nuclease-dead Cas9 is fused to a highly  ...[more]

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