Transcriptomics

Dataset Information

6

Whole rye consumption improves blood and liver n-3 fatty acid profile and gut microbiota composition in rats


ABSTRACT: This study in rats was designed to investigate whether whole rhye (WR) can influence the metabolism of n-3 and n-6 long-chain fatty acids (LCFA) and gut microbiota composition. For 12 weeks, rats were fed a diet containing either 50% WR or 50% refined rye (RR). Total bacterial DNA was extracted from fecal and cecal samples (n=5 per group). 16S PCR amplification was performed to assess the microbial diversity at the family level using the HuGChip. Amplified DNA was purified and labelled with either Cy3 or Cy5 dye and hybridized on the microarray. A 15 chip study was realized, each corresponding to hybridization with 250ng of labelled 16S rRNA gene amplicons from either mice fecal and cecal samples. Each probe (4441) was synthetized in three replicates.

ORGANISM(S): uncultured bacterium  

SUBMITTER: Florence Privé   Luca Calani  William Tottey  Nadia Gaci  Noelia López-Gutiérrez  Fayçal Ounnas  Michel de Lorgeril  Daniele Del Rio  Jean-François Brugère  william tottey  Christine Demeilliers  Florence Hazane-Puch  Patricia Salen  Camilla Melegari  François Laporte  Letizia Bresciani 

PROVIDER: E-GEOD-71726 | ArrayExpress | 2015-08-06

SECONDARY ACCESSION(S): GSE71726PRJNA291983

REPOSITORIES: GEO, ArrayExpress

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