Transcriptomics

Dataset Information

6

Modulation of the transcriptional response to TNF-alpha in the human melanoma cell line MZ7 by MITF


ABSTRACT: We analyzed the transcriptional response of the human melanoma cell line MZ7 to TNF-alpha (24 hours) in a dose-dependent manner (TNF-alpha 10U/ml, 100U/ml, 1000U/ml) either transfected with control siRNA (siNT = non-targeting siRNA) or transfected with siRNAs (pool of 4 active and independent siRNAs) against the melanocytic transcription factor and lineage oncogene MITF. (Microphthalmia-associated transcription factor). The experiment was performed as biological duplicate. As MITF is critical for melanoma cell state control, we aimed to explore how MITF expression intersects with inflammation-induced plasticity pathways in melanoma. Total RNA was obtained from siRNA/TNF-treated MZ7 melanoma cell lines at various conditions and global gene expression profiling was done using the Illumina Human HT12 v4 platform.

ORGANISM(S): Homo sapiens  

SUBMITTER: Michael Holzel   Stefanie Riesenberg  Michael Hölzel 

PROVIDER: E-GEOD-71798 | ArrayExpress | 2015-10-29

SECONDARY ACCESSION(S): GSE71798PRJNA292099

REPOSITORIES: GEO, ArrayExpress

Dataset's files

Source:
Action DRS
E-GEOD-71798.additional.1.zip Other
E-GEOD-71798.idf.txt Idf
E-GEOD-71798.processed.1.zip Processed
E-GEOD-71798.sdrf.txt Txt
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