Genomics

Dataset Information

80

Comparison of genome-wide DNA methylation in MEFs derived from wildtype or estrogen receptor beta null mice using reduced representation bisulfite sequencing


ABSTRACT: Estrogen receptor beta (ERβ) is a ligand inducible transcription factor regulating gene expression in response to the female sex hormone estrogen. Previously, we found that ERβ deficiency results in changes in DNA methylation patterns at two gene promoters, implicating an involvement of ERβ in DNA methylation. In this study, we set out to explore this involvement on a genome-wide level, and to investigate the underlying mechanisms of this function. Using reduced representation bisulfite sequencing (RRBS), we compared genome-wide DNA methylation in mouse embryonic fibroblasts (MEFs) derived from wildtype (wt) and ERβ knock-out (βerko) mice, and identified around 8000 differentially methylated positions (DMPs). This suggests that ERβ is involved in regulating DNA methylation at specific sites in the genome. Genome-wide DNA methylation was analysed in MEFs derived from wildtype and ERbeta null mice by educed representation bisulfite sequencing (RRBS) on an Illumina Genome Analyser IIx platform.

ORGANISM(S): Mus musculus  

SUBMITTER: Nancy Bretschneider  Primo Schär   Yun Liu   Joëlle Rüegg   Mukesh Varshney   Claudia Krawczyk   Gábor Borbély   William Duong   Christian Zinser    

PROVIDER: E-GEOD-72230 | ArrayExpress| 2016-02-27

SECONDARY ACCESSION(S): SRP062668GSE72230PRJNA293446

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Oestrogen receptor β regulates epigenetic patterns at specific genomic loci through interaction with thymine DNA glycosylase.

Liu Yun Y   Duong William W   Krawczyk Claudia C   Bretschneider Nancy N   Borbély Gábor G   Varshney Mukesh M   Zinser Christian C   Schär Primo P   Rüegg Joëlle J  

Epigenetics & chromatin 20160216


DNA methylation is one way to encode epigenetic information and plays a crucial role in regulating gene expression during embryonic development. DNA methylation marks are established by the DNA methyltransferases and, recently, a mechanism for active DNA demethylation has emerged involving the ten-eleven translocator proteins and thymine DNA glycosylase (TDG). However, so far it is not clear how these enzymes are recruited to, and regulate DNA methylation at, specific genomic loci. A number of s  ...[more]

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