Transcriptomics

Dataset Information

41

To identify genes responsive to treatment with the HIF2a inhibitor PT2399


ABSTRACT: HIF2a function is both necessary and sufficient for the growth of VHL-null clear cell Renal Cell Carcinoma (ccRCC). Targeting HIF2a function can therefore be a promising therapeutic strategy. We used microarray analysis to characterize a novel pharmacological inhibitor of HIF2a named PT2399. By comparing genes that are responsive to PT2399 in parental cells vs cells lacking HIF2a, by virtue of CRISPR-mediated genetic editing, we characterized gene signatures that are regulated by PT2399 in a HIF2a dependent manner. Cells were treated with either DMSO (control) or 2uM PT2399 for indicated time periods, total RNA was extracted and analyzed. Please note that the experiments with 786O Parental and HIF2a null cells were conducted independently.

ORGANISM(S): Homo sapiens  

SUBMITTER: Hyejin Cho  William G Kaelin   William G Kaelin Jr   Abhishek A Chakraborty    

PROVIDER: E-GEOD-72959 | ArrayExpress | 2016-12-28

SECONDARY ACCESSION(S): GSE72959PRJNA295413

REPOSITORIES: GEO, ArrayExpress

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