Dataset Information


Gene expression over the course of xylem tracheary element formation in cell suspension cultures of Arabidopsis thaliana

ABSTRACT: Transcriptomic analysis of gene expression during the differentiation of cell suspension cultures into tracheary elements using the biological system published by Pesquet et al., Current Biology (2010): tracheary element differentiation was triggered by externally supplying hormone-free habituated cell suspension cultures of Arabidopsis thaliana Col-0 with auxin, cytokinin and epibrassinolides; RNA samples extracted from 3 independent time-courses every 12h from 0h to 4 days were analyzed using ATH1 Arabidopsis Affymetrix micro-array Time-course design during tracheary element differentiation (3 independepent time-course were harvested from initiation 0h to 4 days every 12h) - total of 27 samples corresponding to the 3 replicates of 9 time point time-courses - gene expression was monitored along the time-course compared to 0h

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Edouard Pesquet  

PROVIDER: E-GEOD-73146 | ArrayExpress | 2015-09-18



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Proteomic Analysis of Microtubule Interacting Proteins over the Course of Xylem Tracheary Element Formation in Arabidopsis.

Derbyshire Paul P   Ménard Delphine D   Green Porntip P   Saalbach Gerhard G   Buschmann Henrik H   Lloyd Clive W CW   Pesquet Edouard E  

The Plant cell 20151002 10

Plant vascular cells, or tracheary elements (TEs), rely on circumferential secondary cell wall thickenings to maintain sap flow. The patterns in which TE thickenings are organized vary according to the underlying microtubule bundles that guide wall deposition. To identify microtubule interacting proteins present at defined stages of TE differentiation, we exploited the synchronous differentiation of TEs in Arabidopsis thaliana suspension cultures. Quantitative proteomic analysis of microtubule p  ...[more]

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