Transcriptomics

Dataset Information

0

Gene expression of canine mammary cancer stem-like cells co-cultured with tumor associated macrophages


ABSTRACT: The aim of our study was to evaluate gene expression of canine mammary cancer stem-like cells co-cultured with tumor associated macrophages. Two canine mammary cancer cell lines (CMT-U27 and P114) were stained using anti-Sca1 (stem cell antigen 1), anti-EpCAM (Epithelial cell adhesion molecule) and anti-CD44 antibody. The FACS analysis showed 0,02-0,05% of Sca1+/EpCAM+/CD44+ in each of the cell line. Cancer stem-like cells were collected using FACS Aria II then co-cultured with tumor associated macrophages and used for further analysis of gene expression ( using Agilent Gene Expression Hybridization Kit ). Canine mammary cancer cell lines were stained using anti-Sca1 (stem cell antigen 1), anti-EpCAM (Epithelial cell adhesion molecule) and anti-CD44 antibodies. Next using FACS Aria II and Sca1+/EpCAM+/CD44+ cells were collected and co-cultured with tumor associated macrophages. Then, total RNA was isolated and hybridized at Gene Expression microarray.

ORGANISM(S): Canis lupus familiaris  

SUBMITTER: J Mucha   A Rybicka  B Taciak  Magdalena Król  M Krol 

PROVIDER: E-GEOD-73230 | ArrayExpress | 2016-06-01

SECONDARY ACCESSION(S): GSE73230PRJNA296756

REPOSITORIES: GEO, ArrayExpress

Similar Datasets

| GSE45622 | GEO
2012-12-07 | E-GEOD-38706 | ArrayExpress
2015-03-03 | E-GEOD-50627 | ArrayExpress
2011-02-01 | E-GEOD-25036 | ArrayExpress
2015-03-16 | E-GEOD-50627 | ExpressionAtlas
2018-01-22 | PXD005782 | Pride
2013-11-11 | E-GEOD-51716 | ArrayExpress
2013-06-20 | E-GEOD-37172 | ArrayExpress
2013-06-13 | E-GEOD-39071 | ArrayExpress
2014-01-24 | E-GEOD-54351 | ArrayExpress