Transcriptomics

Dataset Information

299

Genome-wide analysis of gene expression in MLL translocation transformed mouse leukemia cell lines


ABSTRACT: The purpose of this study is to investigate the transcriptional programs as it relates to disease latency initiated by different MLL fusion proteins, including: MLL-AF1p, MLL-AF6, MLL-Gas7, MLL-AF9 and MLL-ENL. Leukemia cell lines were established by transforming kit+ mouse bone marrow cells with retroviruses coding MLL-AF1p, MLL-AF6, MLL-Gas7, MLL-AF9 or MLL-ENL. At early phase after the cell lines were established, cells growing at exponential phase (cell density at 0.5~1x106/ml) were harvested for RNA extraction and sequencing purpose. Sequencing is performed on total RNA isolated from mouse leukemia cell lines generated from kit+ mouse bone marrow cells transduced with various MLL fusion proteins and is compared to control total RNA isolated from kit+ mouse bone marrow cells.

ORGANISM(S): Mus musculus  

SUBMITTER: Andrew G. Muntean   Andrew G Muntean  Lili Chen  Yuqing Sun  Jingya Wang 

PROVIDER: E-GEOD-73457 | ArrayExpress | 2016-07-07

SECONDARY ACCESSION(S): GSE73457SRP064206PRJNA297051

REPOSITORIES: GEO, ArrayExpress, ENA

altmetric image

Publications

Differential regulation of the c-Myc/Lin28 axis discriminates subclasses of rearranged MLL leukemia.

Chen Lili L   Sun Yuqing Y   Wang Jingya J   Jiang Hui H   Muntean Andrew G AG  

Oncotarget 20160501 18


MLL rearrangements occur in myeloid and lymphoid leukemias and are generally associated with a poor prognosis, however this varies depending on the fusion partner. We modeled acute myeloid leukemia (AML) in mice using various MLL fusion proteins (MLL-FPs) and observed significantly different survival outcomes. To better understand the differences between these leukemias, we examined the genome wide expression profiles of leukemic cells transformed with different MLL-FPs. RNA-sequencing and pathw  ...[more]

Similar Datasets

2018-05-25 | PXD009338 | Pride
| GSE59236 | GEO
2013-04-03 | E-GEOD-43062 | ArrayExpress
| GSE79110 | GEO
2013-04-03 | E-GEOD-43067 | ArrayExpress
2011-04-21 | E-GEOD-19577 | ArrayExpress
| GSE64218 | GEO
2011-04-21 | GSE19577 | GEO
2018-09-17 | E-MTAB-5501 | ArrayExpress
2013-06-30 | E-GEOD-47402 | ArrayExpress