Dataset Information


Transcriptome-wide identification of Hfq-associated RNAs in Brucella suis by deep sequencing

ABSTRACT: We report the identification of new noncoding RNAs in Brucella suis 1330 and that are associated to the chaperone protein Hfq Coimmunoprecipitation using Flag-tagged Hfq as bait

ORGANISM(S): Brucella suis 1330  

SUBMITTER: Alice R Wattam   Philippe Berta  Yanjie Chao  Bashir Saadeh  R Martin Roop II  Clayton C Caswell  David O'Callaghan 

PROVIDER: E-GEOD-73621 | ArrayExpress | 2015-11-25



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Transcriptome-Wide Identification of Hfq-Associated RNAs in Brucella suis by Deep Sequencing.

Saadeh Bashir B   Caswell Clayton C CC   Chao Yanjie Y   Berta Philippe P   Wattam Alice Rebecca AR   Roop R Martin RM   O'Callaghan David D  

Journal of bacteriology 20151109 3

Recent breakthroughs in next-generation sequencing technologies have led to the identification of small noncoding RNAs (sRNAs) as a new important class of regulatory molecules. In prokaryotes, sRNAs are often bound to the chaperone protein Hfq, which allows them to interact with their partner mRNA(s). We screened the genome of the zoonotic and human pathogen Brucella suis 1330 for the presence of this class of RNAs. We designed a coimmunoprecipitation strategy that relies on the use of Hfq as a  ...[more]

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