Transcriptomics

Dataset Information

299

Alternative cleavage and polyadenylation in spermatogenesis coordinates chromatin regulation and post-transcriptional control


ABSTRACT: Most mammalian genes display alternative cleavage and polyadenylation (APA). Previous studies have indicated preferential expression of APA isoforms with short 3’UTRs in testes. Here we show widespread shortening of 3’UTR by APA during the first wave of spermatogenesis in mouse, with 3’UTRs being the shortest in spermatids. Shortening of 3’UTR eliminates destabilizing elements, such as U-rich elements and transposable elements, which appear to be highly potent for transcript elimination during spermatogenesis. We additionally found widespread regulation of APA in introns and global activation of upstream antisense transcripts during spermatogenesis. Interestingly, genes that display 3’UTR shortening tend to have higher levels of H3K4me3, consistent with the open chromatin feature previously observed in spermatids. Since genes with 3’UTR shortening tend to have functions important for further sperm development after spermatids, when transcription is halted, this result indicates that expression of short, stable mRNAs may serve the purpose of mRNA storage for later translation. Thus, APA in spermatogenesis connects regulation of chromatin status with post-transcriptional control, and impacts sperm maturation. 3'READS of 1 week to 6 week of testis development

ORGANISM(S): Mus musculus  

SUBMITTER: Bin Tian   Ghassan Yehia  Mainul Hoque  Wencheng Li  Dinghai Zheng  JiYeon Park 

PROVIDER: E-GEOD-73973 | ArrayExpress | 2016-01-11

SECONDARY ACCESSION(S): SRP064754GSE73973PRJNA298627

REPOSITORIES: GEO, ArrayExpress, ENA

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