Transcriptomics

Dataset Information

297

Next-generation sequencing for the fat mass and obesity-associated protein (FTO) binding mRNA


ABSTRACT: Purpose: The goals of this study are to investigate the mRNAs that bind to the FTO protein in the white fat tissue from mice. Methods: 1. White fat from CAG promoter driven transgenic Flag-tagged FTO mice were extracted with RNA protected. 2. Flag-FTO proteins were immunoprecipitated with control IP using IgG. 3. The immunoprecipitated RNAs were deep sequenced and analyzed. Results: We focused on the mRNAs which have functions related to lipid metabolism and homeostasis. Immunoprecipitated RNAs profiles from Flag-FTO IP and IgG IP were generated by deep sequencing.

ORGANISM(S): Mus musculus  

SUBMITTER: Chao Y Wang   Chao-Yung Wang 

PROVIDER: E-GEOD-74255 | ArrayExpress | 2016-01-05

SECONDARY ACCESSION(S): SRP065222GSE74255PRJNA299573

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Loss of FTO in adipose tissue decreases Angptl4 translation and alters triglyceride metabolism.

Wang Chao-Yung CY   Shie Shian-Sen SS   Wen Ming-Shien MS   Hung Kuo-Chun KC   Hsieh I-Chang IC   Yeh Ta-Sen TS   Wu Delon D  

Science signaling 20151215 407


A common variant of the FTO (fat mass- and obesity-associated) gene is a risk factor for obesity. We found that mice with an adipocyte-specific deletion of FTO gained more weight than control mice on a high-fat diet. Analysis of mice lacking FTO in adipocytes fed a normal diet or adipocytes from these mice revealed alterations in triglyceride metabolism that would be expected to favor increased fatty acid storage by adipose tissue. Mice lacking FTO in adipocytes showed increased serum triglyceri  ...[more]

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