Transcriptomics

Dataset Information

31

Translation State Array Assay for C elegans IFE-1-dependent mRNAs


ABSTRACT: Relative polysomal loading changes for wild type (N2) versus ife-1(bn127) C. elegans strains Strain ife-1(bn127) is null for the gene encoding one of five eIF4E isoforms in the worm, IFE-1. Only this isoform associates with P granules. Resolution of translating and non-translating mRNAs from WT and mutant worms on 10-45% sucrose gradients. Comparision was by ratios of polysomal content. Please note that each sample data table contains raw signal directly imported from the Affymetrix files and the ratio of signals, P/(NP+P), and fold-changes were further calculated. The TSAA_overview.xls contains more datails about the TSAA procedure and 'complete_data.xlsx' contains complete TSAA data output.

ORGANISM(S): Caenorhabditis elegans  

SUBMITTER: Brett D Keiper  Jenna L Hoffman   J K Morrison   Andrew J Friday   Melissa A Henderson    

PROVIDER: E-GEOD-74459 | ArrayExpress| 2016-01-17

SECONDARY ACCESSION(S): GSE74459PRJNA300453

REPOSITORIES: GEO, ArrayExpress

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Publications

Spatial and temporal translational control of germ cell mRNAs mediated by the eIF4E isoform IFE-1.

Friday Andrew J AJ   Henderson Melissa A MA   Morrison J Kaitlin JK   Hoffman Jenna L JL   Keiper Brett D BD  

Journal of cell science 20151105 24


Regulated mRNA translation is vital for germ cells to produce new proteins in the spatial and temporal patterns that drive gamete development. Translational control involves the de-repression of stored mRNAs and their recruitment by eukaryotic initiation factors (eIFs) to ribosomes. C. elegans expresses five eIF4Es (IFE-1-IFE-5); several have been shown to selectively recruit unique pools of mRNA. Individual IFE knockouts yield unique phenotypes due to inefficient translation of certain mRNAs. H  ...[more]

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