Transcriptomics

Dataset Information

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Role of the histone demethylase KDM2B in hematopoietic homeostasis and malignancies [Kras background mouse gene expression]


ABSTRACT: Development of the hematopoietic system is dynamically controlled by the interplay of transcriptional and epigenetic networks to determine cellular identity. Those networks are critical for homeostasis and frequently dysregulated in leukemias. We identified histone demethylase Kdm2b as a critical regulator of definitive hematopoiesis and lineage specification of hematopoietic stem and progenitor cells (HSPCs). RNA sequencing in murine HSPCs and genome-wide chromatin immunoprecipitation studies in human leukemias revealed that Kdm2b regulates differentiation, lineage choice, cytokine signaling, and quiescence. Comparison of gene expression in wild-type and knockout HSPCs We analyzed lineage depleted cells isolated from bone marrow from mice that express oncogenic Kras in the context of knockout and overexpression of Kdm2b in Affymetrxi GeneChip® Mouse Gene 2.0 ST Array

ORGANISM(S): Mus musculus  

SUBMITTER: Yan Kai   Alexandros Tzatsos 

PROVIDER: E-GEOD-74960 | ArrayExpress | 2016-02-22

SECONDARY ACCESSION(S): GSE74960PRJNA302012

REPOSITORIES: GEO, ArrayExpress

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Publications

Histone demethylase KDM2B regulates lineage commitment in normal and malignant hematopoiesis.

Andricovich Jaclyn J   Kai Yan Y   Peng Weiqun W   Foudi Adlen A   Tzatsos Alexandros A  

The Journal of clinical investigation 20160125 3


The development of the hematopoietic system is a dynamic process that is controlled by the interplay between transcriptional and epigenetic networks to determine cellular identity. These networks are critical for lineage specification and are frequently dysregulated in leukemias. Here, we identified histone demethylase KDM2B as a critical regulator of definitive hematopoiesis and lineage commitment of murine hematopoietic stem and progenitor cells (HSPCs). RNA sequencing of Kdm2b-null HSPCs and  ...[more]

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