Transcriptomics,Multiomics

Dataset Information

3

Vibrio cholerae: Wild type El Tor biotype versus crp mutant


ABSTRACT: Transcriptional profiling of an El Tor biotype crp mutant The virulent V. cholerae El Tor Ogawa strain C7258 (Peru isolate 1991) and an isogenic deletion mutant (WL7258) lacking DNA sequences encoding the cAMP receptor protein were grown in LB medium to optical density at 600 nm of 1.5. The cultures were chilled in ice, cells quickly collected by centrifugation and total RNA imediately extracted. RNAwas extracted and purified using the Trizol plus RNA purification system (Invitrogen) followed RNEasy miniElute cleanup (Qiagen). RNA samples were conserved at - 80 C and used within a week. 4 replicates

OTHER RELATED OMICS DATASETS IN: PRJNA99421

ORGANISM(S): Vibrio cholerae  

SUBMITTER: Jorge Antonio Benitez   Alberto P Montano  Weili Liang  Jorge A Benitez  Anisia J Silva 

PROVIDER: E-GEOD-7519 | ArrayExpress | 2010-05-27

SECONDARY ACCESSION(S): GSE7519PRJNA99421

REPOSITORIES: GEO, ArrayExpress

Dataset's files

Source:
Action DRS
E-GEOD-7519.README.txt Txt
E-GEOD-7519.idf.txt Idf
E-GEOD-7519.processed.1.zip Processed
E-GEOD-7519.raw.1.zip Raw
E-GEOD-7519.sdrf.txt Txt
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Publications

The cyclic AMP receptor protein modulates quorum sensing, motility and multiple genes that affect intestinal colonization in Vibrio cholerae.

Liang Weili W   Pascual-Montano Alberto A   Silva Anisia J AJ   Benitez Jorge A JA  

Microbiology (Reading, England) 20070901 Pt 9


Vibrio cholerae is the causative agent of cholera, which continues to be a major public health concern in Asia, Africa and Latin America. The bacterium can persist outside the human host and alternates between planktonic and biofilm community lifestyles. Transition between the different lifestyles is mediated by multiple signal transduction pathways including quorum sensing. Expression of the Zn-metalloprotease haemagglutinin (HA)/protease is subject to a dual regulation which involves the quoru  ...[more]

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